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DOI :
10.3791/54544-v
November 28th, 2016
Chapters
0:05
Title
0:44
Hemolymph Collection to Determine Concentration
1:45
Hemolymph Collection and Immunochemistry
4:32
Larval Lymph Gland Dissection
6:31
Antibody Staining of Larval Lymph Glands
8:32
Mounting Lymph Gland Tissue for Microscopy
9:32
Results: Quantification and Imaging of Hemocytes, and Imaging of Dissected and Immunostained Lymph Gland Tissue
11:19
Conclusion
ショウジョウバエおよび哺乳動物の造血系は、 ショウジョウバエの造血を研究するための魅力的な遺伝モデル作り、多くの共通の特徴を共有します。ここでは、免疫組織化学のための主要な幼虫の造血器官の解剖と取り付けを示しています。また、循環血球と固着液晶セルを含む様々な幼虫の造血コンパートメントをアッセイする方法を記載しています。
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