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DOI :
10.3791/54586-v
•
7:42 min
September 17th, 2016
Chapters
0:05
Title
0:39
Protoplast Isolation
3:16
Protoplast Transfection
5:03
Imaging and Data Analysis
5:48
Results: Detection of Altered Circadian Rhythms in Arabidopsis Protoplasts
7:02
Conclusion
概日時計は、シロイヌナズナのトランスクリプトームの約3分の規制が、計時にフィードバックされる遺伝子の割合は不明のまま。ここでは、急速に一過プロトプラスト内で発現の概日レポーターの発光イメージングを使用して、シロイヌナズナのいずれかの変異体のラインに概日表現型を評価するための方法を視覚化します。
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