Name: using capillary electrophoresis to quantify organic acids from plant tissue: a test case examining coffea arabica seeds
Uploaded: 2016-11-12T14:00:00
Description: Watch this Scientific Journal Video about Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds at JoVE.com

The authors would like to acknowledge the financial support of this project by The J.M. Smucker company.

1. Numune Hazırlama <ol><li> kısa zincirli karboksilik asit (SCCA) çıkarılması için örnekleri birleştirin. işlendikten sonra kalacak yeterli örnek sağlamak için her seferinde kahve tohum 1.0 g hazırlayın. <ol><li> Numuneler önce öğütme işlemi donmuş olsaydı, donma / çözülme hasarı ve örnek oksidasyonunu önlemek için işlem boyunca dondurulmuş doku tutun. taşlama için gerekli yalnızca dondurucu veya sıfırın depolama numuneyi çıkarın. </li><li> taşlama örnek sıvı azot içinde f...

<ol>
	<li>Ara&#250;jo, W. L., Nunes-Nesi, A., Nikoloski, Z., Sweetlove, L. J., Fernie, A. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Metabolic+Control+and+Regulation+of+the+Tricarboxylic+Acid+Cycle+in+Photosynthetic+and+Heterotrophic+Plant+Tissues:+TCA+Control+and+Regulation+in+Plant+Tissues.">Metabolic Control and Regulation of the Tricarboxylic Acid Cycle in Photosynthetic and Heterotrophic Plant Tissues: TCA Control and Regulation in Plant Tissues.</a> Plant Cell Environ. 35 (1), 1-21 (2012).</li><li>Finkemeier, I., Konig, A. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transcriptomic+Analysis+of+the+Role+of+Carboxylic+Acids+in+Metabolite+Signaling+in+Arabidopsis+Leaves.">Transcriptomic Analysis of the Role of Carboxylic Acids in Metabolite Signaling in Arabidopsis Leaves.</a> Plant Physiol. 162 (1), 239-253 (2013).</li><li>Doyle, M. P., Buchanan, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Food Microbiology: Fundamentals and Frontiers. , (2013).</li><li>T&#367;ma, P., Samcov&#225;, E., &#352;tul&#236;k, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determination+of+the+Spectrum+of+Low+Molecular+Mass+Organic+Acids+in+Urine+by+Capillary+Electrophoresis+with+Contactless+Conductivity+and+Ultraviolet+Photometric+Detection-An+Efficient+Tool+for+Monitoring+of+Inborn+Metabolic+Disorders.">Determination of the Spectrum of Low Molecular Mass Organic Acids in Urine by Capillary Electrophoresis with Contactless Conductivity and Ultraviolet Photometric Detection-An Efficient Tool for Monitoring of Inborn Metabolic Disorders.</a> Anal Chim Acta. 685 (1), 84-90 (2011).</li><li>L&#243;pez-Bucio, J., Nieto-Jacobo, M. F., Ram&#305;&#769;rez-Rodr&#305;&#769;guez, V., Herrera-Estrella, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Organic+Acid+Metabolism+in+Plants:+From+Adaptive+Physiology+to+Transgenic+Varieties+for+Cultivation+in+Extreme+Soils.">Organic Acid Metabolism in Plants: From Adaptive Physiology to Transgenic Varieties for Cultivation in Extreme Soils.</a> Plant Sci. 160 (1), 1-13 (2000).</li><li>Cebolla-Cornejo, J., Valc&#225;rcel, M., Herrero-Mart&#236;nez, J. M., Rosell&#242;, S., Nuez, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+Efficiency+Joint+CZE+Determination+of+Sugars+and+Acids+in+Vegetables+and+Fruits:+CE+and+CEC.">High Efficiency Joint CZE Determination of Sugars and Acids in Vegetables and Fruits: CE and CEC.</a> Electrophoresis. 33 (15), 2416-2423 (2012).</li><li>Rosello, S., Galiana-Balaguer, L., Herrero-Martinez, J. M., Maquieira, A., Nuez, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simultaneous+Quantification+of+the+Main+Organic+Acids+and+Carbohydrates+Involved+in+Tomato+Flavour+Using+Capillary+Zone+Electrophoresis.">Simultaneous Quantification of the Main Organic Acids and Carbohydrates Involved in Tomato Flavour Using Capillary Zone Electrophoresis.</a> J Sci Food Agr. 82 (10), 1101-1106 (2002).</li><li>Wasielewska, M., Banel, A., Zygmunt, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Capillary+Electrophoresis+in+Determination+of+Low+Molecular+Mass+Organic+Acids.">Capillary Electrophoresis in Determination of Low Molecular Mass Organic Acids.</a> Int J Environ Sci Dev. 5 (4), 417-425 (2014).</li><li>Galli, V., Garc&#236;a, A., Saavedra, L., Barbas, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Capillary+Electrophoresis+for+Short-Chain+Organic+Acids+and+Inorganic+Anions+in+Different+Samples.">Capillary Electrophoresis for Short-Chain Organic Acids and Inorganic Anions in Different Samples.</a> Electrophoresis. 24 (1213), 1951-1981 (2003).</li><li>Klampfl, C. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determination+of+Organic+Acids+by+CE+and+CEC+Methods.">Determination of Organic Acids by CE and CEC Methods.</a> Electrophoresis. 28 (19), 3362-3378 (2007).</li><li>Kenney, B. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determination+of+Organic+Acids+in+Food+Samples+by+Capillary+Electrophoresis.">Determination of Organic Acids in Food Samples by Capillary Electrophoresis.</a> J Chromatogr A. 546, 423-430 (1991).</li><li>Galli, V., Barbas, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Capillary+Electrophoresis+for+the+Analysis+of+Short-Chain+Organic+Acids+in+Coffee.">Capillary Electrophoresis for the Analysis of Short-Chain Organic Acids in Coffee.</a> J Chromatogr A. 1032 (1-2), 299-304 (2004).</li><li>Schmitt-Kopplin, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Capillary+Electrophoresis:+Methods+and+Protocols.">Capillary Electrophoresis: Methods and Protocols.</a> Methods in Molecular Biology. , 384 (2008).</li><li>Nollet, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Chromatographic analysis of the environment 3rd ed. , (2006).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> ElixerOA Organic Acids/Anions Operating and Instruction Manual, MicroSolv Technology Corperation. , (2001).</li><li>Dahlen, J., Hagberg, J., Karlsson, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+low+molecular+weight+organic+acids+in+water+with+capillary+zone+electrophoresis+employing+indirect+photometric+detection.">Analysis of low molecular weight organic acids in water with capillary zone electrophoresis employing indirect photometric detection.</a> Fresenius J. Anal. Chem. 366 (5), 488-493 (2000).</li><li>Ibanez, A. B., Bauer, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analytical+method+for+the+determination+of+organic+acids+in+dilute+acid+pretreated+biomass+hydrolysate+by+liquid+chromatography-time-of-flight+mass+spectroscopy.">Analytical method for the determination of organic acids in dilute acid pretreated biomass hydrolysate by liquid chromatography-time-of-flight mass spectroscopy.</a> Biotech. For Biofuels. 7 (145), (2014).</li></ol>

herhangi bir analitik tekniği ile olduğu gibi, önemli ölçüde üretilen verilerin kalitesini ve güvenilirliğini etkileyebilir birçok kritik faktör vardır. İlk olarak, donma / erime devirine minimum verimli örnekleri işlemek için önemlidir. Tekrarlanan donma ve çözülme işleme veya analiz öncesi numunenin kimyasal bileşimi bozabilir. İkincisi, sürekli ve eşit tüm örneklerde bu protokolün adımlarını uygulamak için kritik öneme sahiptir. tutarsız numune hazırlama ve işleme kaynaklanan tekn...

Carboxylic acids are organic compounds containing one or more terminal carboxyl functional groups, each attached to an R-group containing one or more carbons (R-C[O]OH). Short chain, low molecular weight carboxylic acids (short chain carboxylic acids, SCCAs) containing between one and six carbons, are essential components of cellular respiration, and function in several biochemical pathways necessary for cell growth and development. SCCAs play critical roles in cellular metabolism1, cell signaling2, and organismal responses to the environment (such as antibiosis3). Because of this, SCCAs can serve as useful indicators of disruptions to cellular metabolism, plant stress responses4,5, and fruit quality6,7. To date, SCCAs have been quantified primarily through chromatographic techniques such as high performance liquid chromatography (HPLC) or gas chromatography-mass spectroscopy (GC-MS). While these methods, are capable of achieving very low limits of detection, they can be expensive, require the derivatization of target SCCAs using caustic and/or toxic reagents, and include lengthy separation runs on the GC or HPLC. Because of this, interest in the use of free zonal capillary electrophoresis (CZE), which does not require sample derivatization, to quantify organic acids has steadily increased8.
Free zonal capillary electrophoresis (CZE) is a chromatographic separation methodology that, due to its high number of theoretical plates, speed, and relative ease-of-use, is increasingly replacing both GC-MS and high-pressure liquid chromatography as an analytical method for the quantification (particularly for quality control purposes) of anions, cations, amino acids, carbohydrates, and short chain carboxylic acids (SCCAs)8,9,10. CZE-based separation of small molecules, including SCCAs, is based two primary principles: the electrophoretic movement of charged ions in an electrical field established across the buffer filling the capillary; and the electro-osmotic movement of the entire buffer system from one end of the capillary to the other, generally towards the negative electrode. In this system, small molecules move towards the negative electrode at varying speeds, with the speed of each molecule determined by the ratio of the net charge of the molecule to the molecular mass. As the movement of each individual molecule in this system is dependent on the charge state of the molecule and the overall rate of electro-osmotic flow (which is itself based on the ion content of the buffer used to fill the capillary), the buffer pH and ionic composition heavily impact the degree to which molecules can be efficiently separated using CZE. Because of this, SCCAs, with their relatively high charge-to-mass ratios, are ideal targets for CZE-based separation. Metabolites separated using CZE can be detected using a variety of methods, including UV absorbance, spectral absorbance (which is generally performed using a photo-diode array [PDA]), and/or mass spectroscopy (CE-MS or CE-MS/MS)8. The diversity of separation and detection methods provided by CZE makes it an extremely flexible and adaptable technique. Because of this, CZE has been increasingly applied as a standard method of analysis in the areas of food safety and quality11,12, pharmaceutical research13, and environmental monitoring13,14. 
Capillary electrophoresis has been used to detect and quantify short chain carboxylic acids for nearly two decades13. The resolving power (particularly for small, charged molecules), short run time, and low per sample cost of CZE analyses make CZE an ideal technique for the separation and quantification of SCCAs13. This method presents a protocol to utilize CZE to measure the concentration of organic acids from plant tissues. Example data was generated through the successful implementation of this protocol to measure the change in organic acid levels in coffee seeds following a secondary fermentation treatment. The protocol details the critical steps and common errors of CZE-based separation of SCCAs, and discusses the means by which this protocol can be successfully applied to quantify SCCAs in additional plant tissues.

<table border="0" cellpadding="0" cellspacing="0" width="782">
	<colgroup>
		<col />
		<col />
		<col />
		<col />
	</colgroup>
	<tbody>
		<tr height="19">
			<td height="19" style="height:19px;width:296px;">Ceramic Moarter and Pestle</td>
			<td style="width:123px;">Coorstek</td>
			<td style="width:119px;">60310</td>
			<td style="width:247px;"></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;width:296px;">Beckman Coulter P/ACE MDQ CE system</td>
			<td style="width:123px;">Beckman Coulter</td>
			<td style="width:119px;">Various</td>
			<td style="width:247px;"></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;width:296px;">Glass sample vials</td>
			<td style="width:123px;">Fisher Inc.</td>
			<td style="width:119px;">033917D</td>
			<td style="width:247px;"></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;width:296px;">1.5 ml microcentrifuge tubes&#160;</td>
			<td style="width:123px;">Fisher Inc.</td>
			<td style="width:119px;">02-681-5</td>
			<td style="width:247px;"></td>
		</tr>
		<tr height="38">
			<td height="38" style="height:38px;width:296px;">LC/MS grade water</td>
			<td style="width:123px;">Fisher Inc.</td>
			<td style="width:119px;">W6-1</td>
			<td style="width:247px;">Milli-Q water (18.2 M&#937;.cm) is also acceptable</td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;width:296px;">15 ml glass tube/ Teflon lined cap&#160;</td>
			<td style="width:123px;">Fisher Inc.</td>
			<td style="width:119px;">14-93331A</td>
			<td style="width:247px;"></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;width:296px;">Parafilm M</td>
			<td style="width:123px;">Fisher Inc.</td>
			<td style="width:119px;">13-374-12</td>
			<td style="width:247px;"></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;width:296px;">CElixirOA detection Kit pH 5.4&#160;</td>
			<td style="width:123px;">MicroSolv</td>
			<td style="width:119px;">06100-5.4</td>
			<td style="width:247px;"></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;width:296px;">BD Safety-Lok syringes</td>
			<td style="width:123px;">Fisher Inc.</td>
			<td style="width:119px;">14-829-32</td>
			<td style="width:247px;"></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;width:296px;">17 mm Target Syringe filter, PTFE</td>
			<td style="width:123px;">Fisher Inc.</td>
			<td style="width:119px;">3377154</td>
			<td style="width:247px;"></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;width:296px;">32 Karat, V. 8.0 control software</td>
			<td style="width:123px;">Beckman Coulter</td>
			<td style="width:119px;">285512</td>
			<td style="width:247px;"></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;width:296px;">capillary electrophoresis (CE) sample vials&#160;</td>
			<td style="width:123px;">Beckman Coulter</td>
			<td style="width:119px;">144980</td>
			<td style="width:247px;"></td>
		</tr>
		<tr height="19">
			<td height="19" style="height:19px;width:296px;">caps for CE vials&#160;</td>
			<td style="width:123px;">Beckman Coulter</td>
			<td style="width:119px;">144648</td>
			<td style="width:247px;"></td>
		</tr>
		<tr height="38">
			<td height="38" style="height:38px;width:296px;">Liquid Nitrogen</td>
			<td style="width:123px;">N/A</td>
			<td style="width:119px;">N/A</td>
			<td style="width:247px;">Liquid nitrogen is available from most facilities services</td>
		</tr>
	</tbody>
</table>

using capillary electrophoresis to quantify organic acids from plant tissue: a test case examining coffea arabica seeds

Karboksilik asitler, bir ya da daha çok terminal karboksil (COOH) veya fonksiyonel gruplar içeren organik asitlerdir. Kısa zincirli karboksilik asitler (merkezler, yerelde, üç ila altı karbon atomu içeren karboksilik asitler) gibi malat ve sitrat gibi, hücresel solunum fonksiyonu ve hücre sağlık göstergesi olarak hizmet birçok biyolojik sistemler, düzgün işlemesi için kritiktir. gıdalar, organik asit içeriği, ekşi veya &quot;asit&quot; terimi tat ile artan SCCA düzeyleri ile tat üzerinde önemli bir etkiye sahip olabilir. Bu nedenle, organik asit, düzeylerinin hızlı analizi için yöntemler yiyecek ve içecek sanayii için özel bir öneme sahiptir. Ancak ne yazık ki, SCCA ölçümü için kullanılan en yöntemler pahalı kromatografik ve / veya kütle spektrometrik analizi ve ardından tehlikeli tepkin maddeler ile örneklerin türetme gerektiren zaman alıcı protokolleri bağlıdır. Bu yöntem org tespiti ve ölçülmesi için alternatif bir yöntem detaylarıBitki materyali ve serbest Kılcal bölge elektroforezi (CZE) ile gıda örnekleri arasında organik asitler, bazen sadece kapiler elektroforez (CE) olarak adlandırılır. CZE algılama düşük limiti (0.005 mg / ml) ile merkezler, yerelde ölçmek için bir düşük maliyetli yöntem sağlar. Bu makalede bitki örneklerinden merkezler, yerelde çıkarılmasını ve miktarının ayrıntıları. Resim yöntemi kahve çekirdeklerinden merkezler, yerelde ölçümü odaklanırken, Resim yöntem birden fazla bitki bazlı gıda maddeleri uygulanabilir.

Bu protokol başarıyla yeşil kahve tohumlarının SCCA içeriğine tohum tedavilerin etkilerini ölçmek için kullanılmıştır. Bu deneyde, altı tedavi edildi: Leuconostoc doymuş bir mikrobiyal süspansiyonu su içinde GCP674 mikropların (1), bir sulu süspansiyon, büyüme ortamı içinde streyn GCP674 pseudomesenteroides (2), asetik asit ve laktik asit (0.15 ve 0.4 arasında bir sulu çözelti mg /), sırasıyla mL (3), bir harcanan M1 büyüme ortamı işleme...

Watch this Scientific Journal Video about Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds at JoVE.com

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Bu makalede, algılama ve serbest Kılcal bölge elektroforezi ile bitki maddesinden organik asitlerin ölçümü için bir yöntem sunulur. Kahve tohumları organik asit seviyesi üzerinde ikincil bir fermantasyon etkilerini belirleyen bu yöntemin olası bir uygulama, bir örneği temin edilmiştir.

Bitki Doku Organik Asit belirlememize Kılcal Elektroforez kullanma: Bir Test Case incelenmesi<em&gt; Coffea arabica</em&gt; Tohumlar

Carboxylic acids are organic acids containing one or more terminal carboxyl (COOH) functional groups. Short chain carboxylic acids (SCCAs; carboxylic ...

Research

JoVE Journal

Chemistry

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Karboksilik Asitler, Boronik Asitler ve Aminler nitel Kimlik haç fluorophores kullanarak

Molecular cruciforms are X-shaped systems in which two conjugation axes intersect at a central core. If one axis of these molecules is substituted with ...

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Thin-layer Chromatographic TLC Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

İnce tabaka kromatografi (TLC) Ayırımı ve bitki özleri Biyodenemeler Antimikrobiyal Bileşikler tanımak

A common screen for plant antimicrobial compounds consists of separating plant extracts by paper or thin-layer chromatography (PC or TLC), exposing the ...

The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy

Hamilelik sırasında Sıçan Karaciğer dokusunda yağ asitleri arasında kompozisyon değişiklikleri analiz Gaz Kromatografi Kullanımı

Gas chromatography (GC) is a highly sensitive method used to identify and quantify the fatty acid content of lipids from tissues, cells, and plasma/serum, ...

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry

Soğuk Giriş Sistemi ve Electron Impact Kütle Spektrometresi kullanarak Uçucu ve Oksidasyon Hassas Bileşiklerin Analizi

This video presents a protocol for the mass spectrometrical analysis of volatile and oxidation sensitive compounds using electron impact ionization. The ...

Synthesis and Characterization of Functionalized Metal-organic Frameworks

Fonksiyonlu Sentezi ve Karakterizasyonu Metal-organik Altyapıları

Metal-organic frameworks have attracted extraordinary amounts of research attention, as they are attractive candidates for numerous industrial and ...

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Kapiler Elektroforez Real Time Kitosan Films üzerine Peptit Aşılama Monitör

Free-solution capillary electrophoresis (CE) separates analytes, generally charged compounds in solution through the application of an electric field. ...

Grafting Multiwalled Carbon Nanotubes with Polystyrene to Enable Self-Assembly and Anisotropic Patchiness

Multiwalled karbon nanotüpler kendinden montajlı etkinleştirmek için polistiren ve Anizotropik seçimdeki aşılama

We demonstrate a straightforward protocol to graft pristine multiwalled carbon nanotubes (MWCNTs) with polystyrene (PS) chains at the sidewalls through a ...

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds

Çevrimsel Voltammetry, UV-VIS-NIR ve EPR Spectroelectrochemistry organik bileşikleri analiz kullanarak

Cyclic voltammetry (CV) is a technique used in the analysis of organic compounds. When this technique is combined with electron paramagnetic resonance ...

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog Xenopus laevis Embryos

Microprobe kılcal Elektroforez kütle spektrometresi canlı Kurbağa (Xenopus laevis) embriyo tek hücreli Metabolomics için

The quantification of small molecules in single cells raises new potentials for better understanding the basic processes that underlie embryonic ...

Microfluidic Preparation of Liquid Crystalline Elastomer Actuators

Sıvı kristal Elastomer aktüatörler mikrosıvısal hazırlanması

This paper focuses on the microfluidic process (and its parameters) to prepare actuating particles from liquid crystalline elastomers. The preparation ...