The Ohio State University, OARDC

1 ARTICLES PUBLISHED IN JoVE

Chemistry

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Michael Joe Vaughan^1,2, Ann Chanon³, Joshua J. Blakeslee^1,3,4

¹Center for Applied Plant Sciences, The Ohio State University, OARDC, ²Department of Plant Pathology, The Ohio State University, OARDC, ³Department of Horticulture and Crop Science, The Ohio State University, OARDC, ⁴OARDC Metabolite Analysis Cluster, The Ohio State University, OARDC

This article presents a method for the detection and quantification of organic acids from plant material using free zonal capillary electrophoresis. An example of the potential application of this method, determining the effects of a secondary fermentation on organic acid levels in coffee seeds, is provided.