Name: rapid acquisition of 3d images using high-resolution episcopic microscopy
Uploaded: 2016-11-21T13:00:00
Description: Watch this Scientific Journal Video about Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy at JoVE.com

We thank Drs. Yichen Huang, Chunming Guo and Zhenfang Zhou for their technical support. This research was funded by NIH/NIDDK (1R01DK091645-01A1, XL) and American Heart Association (AHA, 13GRNT16950006, XL).

すべての動物の使用は、ボストン小児病院で施設内動物管理使用委員会によって承認されています。 1.試料の調製<ol><li> 時限交配 <ol><li>同じケージで一緒のC57Bl6野生型のオスとメスを入れます。 </li><li>次の日の朝早く、嵌合プラグの形成のために確認してください。嵌合プラグ（ 別名 、膣または交尾プラグ）は、そのブロック膣を硬化ゲル状の堆?...

<ol>
	<li>Gregg, C. L., Butcher, J. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+in+vivo+imaging+of+embryonic+development:+opportunities+and+challenges.">Quantitative in vivo imaging of embryonic development: opportunities and challenges.</a> Differentiation. 84, 149-162 (2012).</li><li>Liu, X., Tobita, K., Francis, R. J., Lo, C. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+techniques+for+visualizing+and+phenotyping+congenital+heart+defects+in+murine+models.">Imaging techniques for visualizing and phenotyping congenital heart defects in murine models.</a> Birth defects Res C. 99, 93-105 (2013).</li><li>Norris, F. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+coming+of+age:+advanced+imaging+technologies+for+characterising+the+developing+mouse.">A coming of age: advanced imaging technologies for characterising the developing mouse.</a> Trends Genet. 29, 700-711 (2013).</li><li>Weninger, W. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phenotyping+structural+abnormalities+in+mouse+embryos+using+high-resolution+episcopic+microscopy.">Phenotyping structural abnormalities in mouse embryos using high-resolution episcopic microscopy.</a> Dis model mech. 7, 1143-1152 (2014).</li><li>Mohun, T. J., Weninger, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Episcopic+three-dimensional+imaging+of+embryos.">Episcopic three-dimensional imaging of embryos.</a> Cold Spring Harb protoc. , 641-646 (2012).</li><li>Sizarov, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+and+molecular+analysis+of+the+arterial+pole+of+the+developing+human+heart.">Three-dimensional and molecular analysis of the arterial pole of the developing human heart.</a> J Anat. 220, 336-349 (2012).</li><li>Anderson, R. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Normal+and+abnormal+development+of+the+intrapericardial+arterial+trunks+in+humans+and+mice.">Normal and abnormal development of the intrapericardial arterial trunks in humans and mice.</a> Cardiovasc Res. 95, 108-115 (2012).</li><li>Huang, Y. C., Chen, F., Li, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clarification+of+mammalian+cloacal+morphogenesis+using+high-resolution+episcopic+microscopy.">Clarification of mammalian cloacal morphogenesis using high-resolution episcopic microscopy.</a> Dev Biol. 409, 106-113 (2016).</li><li>Mohun, T. J., Weninger, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Embedding+embryos+for+episcopic+fluorescence+image+capturing+(EFIC).">Embedding embryos for episcopic fluorescence image capturing (EFIC).</a> Cold Spring Harb protoc. , 675-677 (2012).</li><li>Weninger, W. J., Mohun, T. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+analysis+of+molecular+signals+with+episcopic+imaging+techniques.">Three-dimensional analysis of molecular signals with episcopic imaging techniques.</a> Methods mol biol. 411, 35-46 (2007).</li><li>Richardson, D. S., Lichtman, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clarifying+Tissue+Clearing.">Clarifying Tissue Clearing.</a> Cell. 162, 246-257 (2015).</li><li>Hama, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Scale:+a+chemical+approach+for+fluorescence+imaging+and+reconstruction+of+transparent+mouse+brain.">Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain.</a> Nat Neurosci. 14, 1481-1488 (2011).</li><li>Susaki, E. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Whole-brain+imaging+with+single-cell+resolution+using+chemical+cocktails+and+computational+analysis.">Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis.</a> Cell. 157, 726-739 (2014).</li><li>Mohun, T. J., Weninger, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Generation+of+volume+data+by+episcopic+three-dimensional+imaging+of+embryos.">Generation of volume data by episcopic three-dimensional imaging of embryos.</a> Cold Spring Harb protoc. , 681-682 (2012).</li></ol>

ここでは、迅速な3D可視化および複雑な構造の形態計測分析のために互換性のあるシリアルHREM画像を取得するために、ルーチン実験装置を使用して修正されたプロトコルを提示します。高解像度の画像ではなく、個々のセクションのブロック面から直接取得されるため、微細な形態学的特徴を保持し、迅速に3次元可視化プログラムでデジタル再構築されます。 3Dイメー?...

The advent of 3D imaging technologies has opened the possibility of systemic analysis of detailed morphological features during fetal development. A variety of 3D imaging modalities is now available include micro-magnetic resonance imaging (micro-MRI), micro computed tomography, high frequency ultrasound, optical coherence tomography, optical project tomography and episcopic microscopy1-4. Each modality has its own unique features in resolution, contrast, speed, cost, in utero capability and availability.
Episcopic fluorescence image capture (EFIC) and high-resolution episcopic microscopy (HREM) are episcopic microscopy imaging methods4. Here, high-resolution serial images are captured continuously from the block face instead of tissue sections. The resulting images faithfully reflect detailed morphological features with minimal or no tissue distortion. The acquired volumetric data is readily converted to high-resolution 3-D images suitable for accurate morphometric analysis. Because of relative low cost and high resolution, HREM and EPIC have become the superior alternatives for systemic assessment of mouse models of human birth defects2,4-8.
Both EFIC and HREM methods require specimens to be embedded in the suitable medium. EFIC detects autofluorescence emitted from the embedded tissue. Because of this, it is limited to specimens emitting high levels of autofluorescence but not those with relatively weak autofluorescence such as early stage embryos9. To overcome the dependency of autofluorescence, specimens are stained with a fluorescent dye such as eosin for HREM imaging. The choices of dyes and staining methods also make HREM more compatible to detect molecular signals in the context of tissue architecture and morphology4,10.
In this article, we present an improved HREM protocol suitable for whole body assessment of the late stage mouse embryos. To facilitate staining, we include a pretreatment step to increase tissue penetration, thereby enabling HREM imaging of older mouse embryos.

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			<td style="width:356px;">Dell T7600, 2x900GB SAS hard drive and 64 GB DDR3 RDIMM memory</td>
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			<td style="width:356px;">VWR</td>
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			<td height="21" style="height:21px;width:377px;">Analytical Balance</td>
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		</tr>
	</tbody>
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rapid acquisition of 3d images using high-resolution episcopic microscopy

High-resolution episcopic microscopic (HREM) technology enables rapid acquisition of high-resolution digital volumetric and three-dimensional (3D) morphometric data. Here, we describe the detailed protocol to image the entire mouse embryo. The protocol consists of four major sections: sample preparation, embedding, image acquisition and finally, 3D visualization. The technology requires specimens to be stained with a fluorescent dye, which can be problematic for large or dense specimens. To overcome this limitation, we have improved the existing protocol to enhance tissue penetration of the dye by pretreating the specimen with a solution containing urea and sodium dodecyl sulfate. The protocol uses only routine laboratory equipment and reagents for easy adaptation in standard laboratory settings. We show that the resulting high-resolution 3D images faithfully recapitulate the detailed morphologic features of the internal organs of mouse embryos, thereby permitting morphometric analyses. Together, we present a detailed and improved protocol using standard laboratory equipment to acquire high-resolution 3D images of small and large sized specimens.

我々は、E9.5とE13.5 8間のマウス胚の高品質のHREM画像を報告しています。後期段階の胚の画像品質は、しかしながら、かなりために蛍光色素エオシンの限られた組織浸透の危険にさらされています。染色効率を向上させるために、我々は、蛍光イメージング11に互換性のあるいくつかの前処理方法をテストしています。具体的には、E15.5マウス胚のSca /電...

Watch this Scientific Journal Video about Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy at JoVE.com

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

We describe a detailed protocol using high-resolution episcopic microscopy to acquire three-dimensional (3D) images of mouse embryos. This improved protocol utilizes a modified tissue preparation method to enhance penetration of the fluorescent dye, thereby permitting morphometric analysis of both small and large-sized specimens.

高解像度落射顕微鏡を用いた3次元画像の迅速な取得

High-resolution episcopic microscopic (HREM) technology enables rapid acquisition of high-resolution digital volumetric and three-dimensional (3D) ...

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Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

等方性光シート顕微鏡と自動細胞系譜分析による、細胞内分解能を Caenorhabditis elegans 胚発生

Caenorhabditis elegans (C. elegans) stands out as the only organism in which the challenge of understanding the cellular origins of an entire nervous ...

Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

内皮コロニー形成細胞と間葉系幹細胞によって形成された共培養スフェロイドを用いた3次元血管新生アッセイ系

Studies in the field of angiogenesis have been aggressively growing in the last few decades with the recognition that angiogenesis is a hallmark of more ...

Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy

ブライトフィールド顕微鏡を用いた幼虫ゼブラフィッシュの肝臓サイズの定量化

In several transgenic zebrafish models of hepatocellular carcinoma (HCC), hepatomegaly can be observed during early larval stages. Quantifying larval ...

Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

全実装免疫学化学と3D再構成を用いた咽頭弓動脈の可視化と解析

Improper formation or remodeling of the pharyngeal arch arteries (PAAs) 3, 4, and 6 contribute to some of the most severe forms of congenital heart ...

4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy

4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy

4D顕微鏡:ノマルスキ顕微鏡を用いた カエノラブディティス・エレガンス 胚発生の解明

4D microscopy is an invaluable tool for unraveling the embryonic developmental process in different animals. Over the last decades, Caenorhabditis elegans ...