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DOI :
10.3791/54630-v
October 19th, 2016
Chapters
0:05
Title
0:55
Preparing the Mouse and Isolation of the Aorta
2:06
Preparing Tissue Samples for the AFM Measurements
2:46
Loading the AFM Probe and Aligning the Laser
4:16
Calibrating the Deflection Sensitivity and Spring Constant of the AFM Probe
6:28
Measuring the Elastic Modulus on Mouse Arteries Ex Vivo
7:50
Data Analysis
8:47
Results: Determining Aortic Stiffness
9:29
Conclusion
We present detailed protocols for isolation of aortas from mouse and measurement of their elastic modulus using atomic force microscopy.
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