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DOI :
10.3791/54640-v
October 21st, 2016
Chapters
0:05
Title
0:36
SBDS-FlAsH Tag Protein Expression and Purification
4:10
Elongation Factor Like-1 (EFL1) Protein Expression and Purification
7:07
Labeling of SBDS-FlAsH with the FlAsH Fluorescent Dye 4',5'-Bis(1,3,2 dithioarsolan-2-yl) Fluorescein and Fluorescence Anisotropy Experiments
8:50
Results: Evaluation of Protein-protein Interactions Using Fluorescence Anisotropy
9:55
Conclusion
蛋白质相互作用是在一个细胞的功能的心脏。量热和光谱技术通常用于表征它们。这里我们介绍荧光各向异性作为一种工具来研究在Shwachman-Diamond综合征(SBDS)突变蛋白质和延伸因子样1 GTP酶(EFL1)之间的相互作用。
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