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DOI :
10.3791/54640-v
October 21st, 2016
Chapters
0:05
Title
0:36
SBDS-FlAsH Tag Protein Expression and Purification
4:10
Elongation Factor Like-1 (EFL1) Protein Expression and Purification
7:07
Labeling of SBDS-FlAsH with the FlAsH Fluorescent Dye 4',5'-Bis(1,3,2 dithioarsolan-2-yl) Fluorescein and Fluorescence Anisotropy Experiments
8:50
Results: Evaluation of Protein-protein Interactions Using Fluorescence Anisotropy
9:55
Conclusion
タンパク質相互作用は、細胞の機能の中心にあります。熱量分光技術は、一般に、それらを特徴付けるために使用されます。ここでは、Shwachman-ダイヤモンド症候群(SBDS)で変異タンパク質と伸長因子様1 GTPアーゼ(EFL1)間の相互作用を研究するためのツールとして蛍光異方性を説明します。
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