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Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

DOI :

10.3791/54641-v

November 4th, 2016

November 4th, 2016

11,620 Views

1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, 2Flow Cytometry Laboratory, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University

We report a protocol using fluorescence-activated cell sorting to isolate plasmacytoid dendritic cells (pDC) with high purity from the bone marrow of lupus-prone mice for functional studies of pDC.

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