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DOI :
10.3791/54652-v
November 23rd, 2016
Chapters
0:05
Title
1:11
Preparation of 2-pyridyl Undec-10-ynyl Carbonate and Undec-10-ynyl 2-oxopyridine 1-carboxylate
4:17
Click Chemistry with Azide-PEG3-Biotin
5:14
Removal of Excess Click Chemistry Reagents
6:29
Streptavidin Enrichment
7:57
Click Chemistry with Azide-PEG3-Fluor 545 on Tissue Slices
8:59
Results: Synthesis and Applications of NAAA Activity Based Probe, ARN14686
10:21
Conclusion
2:44
Preparation of Undec-10-ynyl-N-[(3S)-2-oxoazetidin-3-yl]carbamate
ここでは、アクティビティベースのプローブの調製および使用を記載する(ARN14686、ウンデカ-10- ynyl- N - [(3 S)-2-オキソアゼチジン-3-イル]カルバメート)の検出および定量を可能にします前炎症性酵素Nの活性形態は、in vitroおよびex vivoの両方、酸アミダーゼ(NAAA)を-acylethanolamine。
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