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본래 마우스 독도에 β 세포 분열 촉진 물질의 지속적인 관리

DOI :

10.3791/54664-v

November 5th, 2016

November 5th, 2016

7,157 Views

1Department of Medicine, Vanderbilt University Medical Center, 2School of Engineering, Vanderbilt University, 3Department of Veterans Affairs, Tennessee Valley Health Authority

Here, we present methodology to generate and administer compound of interest-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres to intact mouse islets in culture with subsequent immunofluorescence analysis of β-cell proliferation. This method is suitable for determining the efficacy of candidate β-cell mitogens.

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