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DOI :
10.3791/54667-v
March 29th, 2017
Chapters
0:05
Title
1:14
Isolation of Human Blood-derived Neutrophils by Density Gradient Centrifugation
3:59
Isolate the Lipids from the Human Peripheral Blood-derived Neutrophils
5:33
High Performance Thin Layer Chromatography (HPTLC) to Semi-quantify the Amount of Lipids
7:53
High Performance Liquid Chromatography (HPLC) to Quantify the Amount of Cholesterol
8:48
Results: Isolation of Human Blood-derived Neutrophils and Subsequent Lipid Analysis
9:33
Conclusion
지질은 세포 기능에 중요한 역할을하는 것으로 알려져있다. 여기, 우리는 호중구 세포 트랩 형성의 기본 메커니즘의 더 나은 이해를 얻기 위해 HPTLC 및 HPLC를 모두 사용하여, 콜레스테롤 수준에 중점을두고, 호중구의 지질 구성을 결정하는 방법을 설명합니다.
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