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DOI :
10.3791/54670-v
November 2nd, 2016
Chapters
0:05
Title
0:41
Preparation and Dissection of Larvae
2:34
Fixation and Mounting of Larval Fillets
4:10
Results: Muscle Removal Enables Enhanced Imaging of Da Neurons and the Epidermis
6:02
Conclusion
ショウジョウバエの幼虫の樹状樹枝状(DA)ニューロンを用いて、神経細胞の形態形成の研究は、免疫蛍光によって、神経や表皮タンパク質のその場可視化で恩恵を受ける。私たちは、幼虫の体壁から筋肉組織を除去することにより、ダニューロンと周囲の表皮細胞の免疫蛍光分析を向上させる手順を説明します。
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