Conformaciones GPCR Proteína G-selectivos mide usando sensores FRET en un fluorómetro Ensayo de suspensión de células vivas

Simple methods to detect the selective activation of G proteins by G protein-coupled receptors remain an outstanding challenge in cell signaling. Here, Fӧrster resonance energy transfer (FRET) biosensors have been developed by pairwise tethering a GPCR to G protein peptides to probe conformational changes at controlled concentrations in live cells.