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DOI :
10.3791/54697-v
October 25th, 2016
Chapters
0:05
Title
0:35
T Cell Receptor (TCR) Hemichain-encoded Retroviral Construct Preparation
1:41
Stable Packaging Cell Line Generation
3:08
Human T Cell Activation and Transduction
5:04
Novel Antigen-specific TCR Clone Reconstitution
6:25
Results: Centric TCR Hemichains Pair with Different Counter-chains to Form Antigen-specific TCRs with Varying Affinity
7:59
Conclusion
这里我们描述了一种新方法,以产生抗原特异性T细胞受体(TCR)通过配对现有的TCR的TCRα或TCRβ,具有感兴趣的抗原特异性,与外周T细胞受体库的补充hemichain。 从头产生的TCR保留抗原特异性有不同的亲和力。
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