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DOI :
10.3791/54709-v
November 8th, 2016
Chapters
0:05
Title
1:05
Detection of Endogenous SCAP Protein in Human Cells
4:11
Analysis of SCAP N-glycosylation in Human Cells
7:15
Detection of SCAP Trafficking from ER to Golgi Using GFP-SCAP in Human Cells
9:07
Results: SCAP Trafficking from ER to Golgi
10:43
Conclusion
我们通过使用蛋白质印迹描述了从人类细胞的膜级分的分离和样品制备用于检测SCAPÑ-glycosylation和总蛋白的改进方法。我们还引进了GFP标记法使用共聚焦显微镜监测SCAP贩卖。这种协议可以在常规生物学实验室使用。
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