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DOI :
10.3791/54718-v
•
11:36 min
November 20th, 2016
Chapters
0:05
Title
0:58
Preparation of FRT-containing hPSC Line for Transfection
4:44
Transfection by Nucleofection
6:28
Positive and Negative Selection of Cells Undergoing RMCE
7:28
Expansion and Characterization of RMCE Lines
8:31
Results: RMCE in the AAVS1 of hPSC
10:51
Conclusion
这里,我们报告在先前所描述的系统,提高了在人多能干细胞(hPSCs)的AAVS1轨迹基于RMCE迅速和有效的基因编辑方法。使用这种技术,基因系可迅速而可靠地进行适当的比较研究产生,促进与hPSCs转基因介导的研究。
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