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DOI :
10.3791/54718-v
•
11:36 min
November 20th, 2016
Chapters
0:05
Title
0:58
Preparation of FRT-containing hPSC Line for Transfection
4:44
Transfection by Nucleofection
6:28
Positive and Negative Selection of Cells Undergoing RMCE
7:28
Expansion and Characterization of RMCE Lines
8:31
Results: RMCE in the AAVS1 of hPSC
10:51
Conclusion
ここでは、以前に記載されたシステムを改良するヒト多能性幹細胞(hPSCs)のAAVS1座にRMCEに基づいて、迅速かつ効率的な遺伝子編集方法を報告しています。この技術を用いて、同質遺伝子系統を迅速かつ確実にhPSCsで形質転換媒介性の研究を容易に、適切な比較試験のために生成することができます。
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