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DOI :
10.3791/54774-v
December 1st, 2016
Chapters
0:05
Title
0:55
Imaging Specimen Preparation
3:11
Sample Placement and iPALM Alignment
5:29
Calibration of the iPALM Setup
7:47
Data Acquisition
9:09
Results: Actin Cytoskeleton of Adherent Mammalian Cells Visualized by iPALM with Sub-20 nm Spatial Resolution in 3D
10:55
Conclusion
我们提出了一个协议,用于干涉式光活化的定位显微镜(iPALM),3维单分子定位超分辨率显微镜方法的应用,以便在贴壁哺乳动物细胞的肌动蛋白细胞骨架的成像。这种方法允许的纳米结构特征,否则将通过常规的衍射限制的光学显微镜仍未解决基于光的可视化。
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