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DOI :
10.3791/54776-v
December 4th, 2016
Chapters
0:05
Title
1:10
Preparation of the DOTA-type Macrocycle Monomer
3:05
Preparation of the Dendrimeric Chelator
4:55
Preparation of the Gd(III) Dendrimeric Constrast Agent (DCA)
6:28
In Vitro Characterization of a Dendrimeric Contrast Agent (DCA) and Monomeric Analogue (GdDOTA)
9:09
Results: Dendrimeric Contrast Agent Activity
10:38
Conclusion
このプロトコルは、常磁性のガドリニウムイオンを配位シクレンベースの大環状キレートを担持デンドリマー磁気共鳴イメージング(MRI)造影剤の調製および特徴付けを記載します。市販の単量体のアナログと比較した場合、in vitroでのMRI一連の実験では、このエージェントは、増幅されたMRI信号を生成しました。
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