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DOI :
10.3791/54800-v
•
8:25 min
December 7th, 2016
Chapters
0:05
Title
0:51
Embryonic Heart Dissection and Fixation
2:07
Embryonic Heart Whole Mount Immunostaining
5:25
Results: Representative Embryonic Heart Imaging
7:11
Conclusion
我々は、光のクリアランス、共焦点顕微鏡に続く標準的な免疫学的染色法を用いて、E15.5までの全胚マウス心臓における冠血管の分析のためのプロトコルを提示します。この技術は、連続切片の時間のかかる分析を必要とすることなく、心臓全体にわたって血管の可視化を可能にします。
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