UCL Institute of Child Health

3 ARTICLES PUBLISHED IN JoVE

Behavior

Cortical Source Analysis of High-Density EEG Recordings in Children

Joe Bathelt¹, Helen O'Reilly², Michelle de Haan¹

¹Cognitive Neuroscience & Neuropsychiatry Section, UCL Institute of Child Health, ²Academic Division of Neonatology, Institute for Women's Health, University College London

In recent years, there has been increasing interest in estimating the cortical sources of scalp measured electrical activity for cognitive neuroscience experiments. This article describes how high density EEG is acquired and how recordings are processed for cortical source estimation in children from the age of 2 years at the London Baby Lab.

Developmental Biology

Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick^GFPNeural Tube Grafting and Carbocyanine Dye DiI Injection

Jean-Marie Delalande^1,2, Nikhil Thapar¹, Alan J. Burns^1,3

¹Birth Defects Research Centre, UCL Institute of Child Health, ²Blizard Institute, Centre for Digestive Diseases, Queen Mary University of London, Barts and The London School of Medicine and Dentistry, ³Department of Clinical Genetics, Erasmus University Medical Center, Rotterdam

Here we report dual labeling of neural crest cells and blood vessels using chick^GFPneural tube intraspecies grafting combined with intra-vascular DiI injection. This experimental technique allows us to simultaneously visualize and study development of the NCC-derived (enteric) nervous system and the vascular system, during organogenesis.

Developmental Biology

Analysis of Coronary Vessels in Cleared Embryonic Hearts

Sarah Ivins¹, Catherine Roberts¹, Bertrand Vernay², Peter J. Scambler¹

¹Developmental Biology of Birth Defects, UCL Institute of Child Health, ²MRC Centre for Regenerative Medicine, SCRM Building, University of Edinburgh

We present a protocol for the analysis of coronary vessels in whole embryonic murine hearts up to E15.5, using standard immunological staining methods followed by optical clearance and confocal microscopy. This technique enables visualization of blood vessels throughout the entire heart without the need for time-consuming analysis of serial sections.