Name: mouse microsurgery infusion technique for targeted substance delivery into the cns via the internal carotid artery
Uploaded: 2017-01-31T14:00:00
Description: Watch this Scientific Journal Video about Mouse Microsurgery Infusion Technique for Targeted Substance Delivery into the CNS via the Internal Carotid Artery at JoVE.com

We would like to thank Dr. Lei Chen (Icahn School of Medicine at Mount Sinai, NY) who first established the use of this model in our laboratory, and to Dr. Gretchen Wolff (German Cancer Research Center, Heidelberg, Germany) for disseminating the technique in our laboratory. Supported in part by HL126559, DA039576, MH098891, MH63022, MH072567, DA027569, and NSC 2015/17/B/NZ7/02985.

Aşağıdaki protokolde yer alan işlemler Miami Kurumsal Hayvan Bakımı ve Kullanımı Komitesi Üniversitesi (IACUC) tarafından onaylanmıştır. Buna ek olarak, tüm işlemler laboratuvar Animal Care International Değerlendirme ve Akreditasyon Derneği (AAALAC) tarafından onaylanan tesislerinde yürütülmektedir. Cerrahi Farelerin 1. Hazırlık <ol><li> Bir laboratuvar anestezi sistemi kullanılarak, oksijen ile karışık izofluran ile fare anestezisi. 2 L% ve oksijen akışı 4-5...

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Burada anlatılan infüzyon mikrocerrahi gövdesi 1, 12 boyunca istenmeyen yayılmasını önleyerek, CNS içine çeşitli biyolojik özellikleri eksojen maddelerin teslim çok başarılı olduğu kanıtlanmıştır. Kan-beyin bariyerinin bozulması çok merkezi sinir sistemi ile ilgili hastalıkların patolojik özelliğidir; Bu nedenle, kan-beyin bariyeri ile ekzojen maddelerin ilişkisini değerlendirmek büyük önem ve ilgi konusudur. <p class="jove_con...

Merkezi sinir sisteminin in vivo modellerde (CNS) hastalıkları, beyin bu tür ilaçlar, patojenlerin veya eksozom gibi eksojen madde, etkili bir teslim gerektirir. Bu nedenle, ideal bir dağıtım yöntemi, hayvana en az travmaya neden nöronal ağ bütünlüğünü korumak ve beyin 1 yüksek madde konsantrasyonları elde etmelidir. Yerel madde sağlama çeşitli cerrahi yöntem içi kılıf, intraserebral, intraventriküler enjeksiyonlar veya implantlar, 2, 3, 4, 5 de dahil olmak üzere tarif edilmiştir. Bu yaklaşımlar, ancak, CNS travmatik kabul ve ilgi maddenin sadece düşük hacimli yönetimine izin vardır. Ayrıca, dış kaynaklı maddelerin hızlı bir şekilde beyin omurilik sıvısı 6 çıkarılabilir ileri sürülmüştür </skadar&gt;, ve yukarıda belirtilen teknikler kullanıldığı zaman beyin parankiması için düşük penetrasyon aralığı 7 gözlenmiştir. Bunlar merkezi sinir sistemine maddeleri sunan düşük etkinliği sergileyen ancak örneğin oral, akciğer, deri altı ve damar içi yollar gibi sistemik uygulama yöntemleri, daha yaygın olarak diğer organlarda 8, 9 alımı nedeniyle, hayvan modelleri kullanılmaktadır. Bu nedenle, teslimat bu rotalar yan etki ve toksisite 10, 11 riskini artıran, yönetilen maddelerin yüksek dozda gerektirir. Burada, etkili doğrudan internal karotid arter yoluyla beyine maddeleri sağlayan bir fare infüzyon mikrocerrahi tekniği, açıklar. CNS teslimat hedeflemenin yanı sıra, bu teknik, normal fizyolojik engelleri aşmak ve bu nedenle biologica son derece alakalı değilbeyne terapötik veya patojenlerin pasajlar yer l süreçleri.

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			<td height="21" style="height:21px;width:367px;">Nylon suture</td>
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			<td height="21" style="height:21px;width:367px;">2,3,5-Triphenyltetrazolium chloride</td>
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mouse microsurgery infusion technique for targeted substance delivery into the cns via the internal carotid artery

Animal models of central nervous system (CNS) diseases and, consequently, blood-brain barrier disruption diseases, require the delivery of exogenous substances into the brain. These exogenous substances may induce injurious impact or constitute therapeutic strategy. The most common delivery methods of exogenous substances into the brain are based on systemic deliveries, such as subcutaneous or intravenous routes. Although commonly used, these approaches have several limitations, including low delivery efficacy into the brain. In contrast, surgical methods that locally deliver substances into the CNS are more specific and prevent the uptake of the exogenous substances by other organs. Several surgical methods for CNS delivery are available; however, they tend to be very traumatic. Here, we describe a mouse infusion microsurgery technique, which effectively delivers substances into the brain via the internal carotid artery, with minimal trauma and no interference with normal CNS functionality.

Burada tarif edilen fare infüzyon mikrocerrahi tekniği çok yönlüdür ve beyin metastaz oluşumunun 1, 12 ait temsili bir modelinde tümör hücrelerinin verilmesi de dahil olmak üzere, doğrudan beyin içine farklı maddelerin aktarılması için kullanılmıştır. Bu teknik aynı zamanda CNS farklı patojenlerin patolojik açıdan değerlendirmek için uygundur...

Watch this Scientific Journal Video about Mouse Microsurgery Infusion Technique for Targeted Substance Delivery into the CNS via the Internal Carotid Artery at JoVE.com

Mouse Microsurgery Infusion Technique for Targeted Substance Delivery into the CNS via the Internal Carotid Artery

The present protocol describes a mouse microsurgery infusion technique, which effectively delivers substances directly into the brain via the internal carotid artery.

CNS içine Hedeflenen Madde Teslimat için Fare Mikrocerrahi İnfüzyon Tekniği<i&gt; yoluyla</i&gt; İç Karotis Arter

Animal models of central nervous system (CNS) diseases and, consequently, blood-brain barrier disruption diseases, require the delivery of exogenous ...

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Neuroscience

Mouse Microsurgery Infusion Technique for Targeted Substance Delivery into the CNS via the Internal Carotid Artery

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