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Visualizing the Effects of Sputum on Biofilm Development Using a Chambered Coverglass Model

DOI :

10.3791/54819-v

December 14th, 2016

December 14th, 2016

8,154 Views

1Physiology and Experimental Medicine, Hospital for Sick Children, 2Department of Clinical Microbiology, Royal College of Surgeons in Ireland, 3Department of Laboratory Medicine and Pathobiology, University of Toronto, 4Department of Pediatrics, University of Toronto

This protocol describes the visualization of biofilm development following exposure to host-factors using a slide chamber model. This model allows for direct visualization of biofilm development as well as analysis of biofilm parameters using computer software programs.

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