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DOI :
10.3791/54820-v
December 21st, 2016
Chapters
0:05
Title
1:04
Whole Mount 3β-Hsd Activity Staining
3:15
IHC Analysis of 3β-Hsd Activity-stained Embryos
5:28
Results: Zebrafish Interrenal Steroidogenic Tissue
6:30
Conclusion
ゼブラフィッシュにおける間腎腺は、哺乳動物の副腎の硬骨魚類の対応です。酵素活性アッセイ、開発ゼブラフィッシュでは、分化ステロイド産生細胞を検出し、このプロトコルは、3-βヒドロキシステロイドデヒドロゲナーゼ(3β-HSDΔ5-4イソメラーゼ)を実行する方法を紹介します。
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