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DOI :
10.3791/54845-v
•
10:30 min
December 8th, 2016
Chapters
0:05
Title
0:57
Preparation of Autologous Serum
2:20
Isolation of hMSCs from Bone Marrow
3:42
Culture of hMSCs in the Presence of Autologous Serum
5:19
Osteogenic Pre-induction and Recovery of hMSCs
6:20
Preparation of hMSCs/Fibrin Clot Constructs and Cell Viability Assay
7:30
Results: hMSCs/Fibrin Clot Generation and Application
9:10
Conclusion
培养人骨髓间充质基质细胞(hMSCs)与自体血清,降低排斥异种材料等负面影响的风险。它也允许对中胚层祖细胞的子集,这可以提供新鲜的hMSCs的恢复。在自体纤维蛋白凝块嵌入的hMSCs可轻松处理和有效的手术植入。
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