Name: optical trap loading of dielectric microparticles in air
Uploaded: 2017-02-05T13:00:00
Description: Watch this Scientific Journal Video about Optical Trap Loading of Dielectric Microparticles In Air at JoVE.com

All work performed under the support of the National Institute of Standards and Technology. Certain commercial equipment, instruments, or materials are identified to foster understanding of this protocol. Such identification does not imply recommendation or endorsement by the National Institute of Standards and Technology, nor does it imply that the materials or equipment identified are necessarily the best available for the purpose.

Forsigtig: Se venligst alle relevante sikkerhedskrav programmer, før forsøget. Alle de eksperimentelle procedurer beskrevet i denne protokol udføres i overensstemmelse med NIST LASER sikkerhed program samt andre gældende regler. Sørg for at vælge og bære korrekt personlige værnemidler (PPE) såsom laser beskyttelse briller designet til specifik bølgelængde og magt. Håndtering af tørre nano / mikropartikler kan kræve yderligere åndedrætsværn. 1. Design og fremstilling af en PZT ...

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Den piezoelektriske løfteraket er designet til at optimere den dynamiske udførelsen af ​​en udvalgt PZT. Korrekt valg af PZT materialer og styring af ultralyds vibrationer er de vigtigste skridt til at giver en vellykket eksperiment. PZTs har forskellige egenskaber afhængigt af typen af ​​transducer (bulk eller stablet) og komponent materialer (hårde eller bløde). En bulk-typen PZT fremstillet af et hårdt piezoelektrisk materiale er valgt af følgende grunde. Første, hårde piezoelektriske materialer har ...

Ashkin rapporterede acceleration og indfangning af mikropartikler ved stråling pres i 1970. 1 Hans roman præstation fremmet udviklingen af optiske fældefangst teknikker som et vigtigt værktøj for grundlæggende studier af fysik og biofysik. 2, 3, 4, 5 har til dato anvendelsen af optisk indfangning hovedsageligt fokuseret på flydende miljøer, og er blevet anvendt til at undersøge en meget bred vifte af systemer, fra adfærd kolloider til de mekaniske egenskaber af enkelte biomolekyler. 6, 7, 8 Anvendelse af optisk indfangning til gasformige medier, kræver imidlertid løse en række nye tekniske spørgsmål. For nylig, optisk indfangning i luft / vakuum stigende grad er blevet anvendt i grundforskning. Da optiske levitation potentielt giver næsten-fuldstændig isolation af et system fra det omgivende miljø, det optisk svæve partikel bliver en ideel laboratorium til at studere quantum jorden stater i små genstande, 4 måler højfrekvente gravitationsbølger, 9 og søge efter fraktioneret gebyr. 10 Endvidere er den lave viskositet af luft / vakuum tillader en at bruge inerti til at måle den øjeblikkelige hastighed af en Brownsk partikel 11 og skabe ballistisk bevægelse over et bredt område af bevægelse ud over den lineære fjederlignende regime. 12 Derfor, detaljerede tekniske oplysninger og praksis for optiske fælder i gasformige medier er blevet mere værdifuld for det bredere forskersamfund. Nye eksperimentelle teknikker skal indlæse nano / mikropartikler i optiske fælder i gasformige medier. En piezoelektrisk transducer (PZT), en anordning, der konverterer strømic energi til mekanisk-akustisk energi, er blevet anvendt til at levere små partikler til optiske fælder i luft / vakuum 5, 12 siden den første demonstration af optisk levitation. 1 Siden da er der blevet foreslået flere lastning teknikker til at indlæse mindre partikler ved hjælp af flygtige aerosoler genereret af en kommerciel forstøver 13 eller en akustisk bølge generator. 14 De flydende aerosoler med solide inklusioner (partikler) passerer tilfældigt nær fokus og er fanget ved en tilfældighed. Når aerosolen er fanget, opløsningsmidlet fordamper, og partiklen forbliver i den optiske fælde. Imidlertid er disse metoder ikke velegnede til at identificere ønskede partikler inde fra en prøve, indlæse en valgt partikel og spore dets ændringer ved frigivelse fra fælden. Denne protokol er beregnet til at give detaljer til nye praktikere på selektiv optisk fælde lastning i luften, herunder eksperimentetal opsætning, fremstilling af en PZT holder og prøve kabinet, fælde lastning, og dataopsamling i forbindelse med analysen af ​​partikel bevægelse i både frekvens og tid domæner. Protokoller til fældefangst i flydende medier er også blevet offentliggjort. 15, 16 Den samlede forsøgsopstilling er udviklet på en kommerciel inverteret optisk mikroskop. Figur 1 viser et skematisk diagram af opsætningen anvendes til at vise trin i den selektive optiske fælde loading: frigør hvilende mikropartikler, løfte den valgte partikel med den fokuserede stråle, måle dens bevægelse, og placere den på substratet igen. Først translationelle stadier (tværgående og lodrette), der anvendes til at bringe en udvalgt mikropartikel på underlaget til fokus for en trapping laser (bølgelængde 1064 nm) fokuserede af en objektiv linse (nær-infrarødt korrigeret lange arbejdsafstand mål: NA 0,4, forstørrelse 20X, arbejder distance 20 mm) gennem det transparente substrat. Derefter blev en piezoelektrisk launcher (a mekanisk pre-loaded ring-typen PZT) genererer ultrasoniske vibrationer at bryde vedhæftningen mellem mikropartikler og et substrat. Således kan enhver befriet partikel løftes af den single-beam gradient laser fælde fokuseret på den valgte partikel. Når partiklen er fanget, er det oversat til midten af ​​prøven indelukke indeholdende to parallelle ledende plader til elektrostatisk excitation. Endelig a (DAQ) -system datafangst samtidigt registrerer partikel bevægelse, fanget af en kvadrant-celle fotodetektor (QPD), og det påførte elektriske felt. Efter endt måling partiklen styrbart placeret på substratet, så den igen kan blive fanget i en reversibel måde. Denne samlede proces kan gentages hundredvis af gange uden partikel tab at måle ændringer såsom kontakt elektrificering forekommer over flere fældefangst cyklusser. Se vores seneste artikel feller detaljer. 12

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optical trap loading of dielectric microparticles in air

We demonstrate a method to trap a selected dielectric microparticle in air using radiation pressure from a single-beam gradient optical trap. Randomly scattered dielectric microparticles adhered to a glass substrate are momentarily detached using ultrasonic vibrations generated by a piezoelectric transducer (PZT). Then, the optical beam focused on a selected particle lifts it up to the optical trap while the vibrationally excited microparticles fall back to the substrate. A particle may be trapped at the nominal focus of the trapping beam or at a position above the focus (referred to here as the levitation position) where gravity provides the restoring force. After the measurement, the trapped particle can be placed at a desired position on the substrate in a controlled manner. 
In this protocol, an experimental procedure for selective optical trap loading in air is outlined. First, the experimental setup is briefly introduced. Second, the design and fabrication of a PZT holder and a sample enclosure are illustrated in detail. The optical trap loading of a selected microparticle is then demonstrated with step-by-step instructions including sample preparation, launching into the trap, and use of electrostatic force to excite particle motion in the trap and measure charge. Finally, we present recorded particle trajectories of Brownian and ballistic motions of a trapped microparticle in air. These trajectories can be used to measure stiffness or to verify optical alignment through time domain and frequency domain analysis. Selective trap loading enables optical tweezers to track a particle and its changes over repeated trap loadings in a reversible manner, thereby enabling studies of particle-surface interaction.

Den PZT løfteraket er konstrueret ved hjælp af en CAD-software-pakke. Her, bruger vi en simpel sandwich struktur for forbelastning (a PZT fastspændt med to plader), som vist i figur 2. PZT holder og prøven kabinet kan være fremstillet af en række forskellige materialer og metoder. For en hurtig demonstration, vælger vi 3D-print med termoplast som illustreret i figur 2d. Baseret på de fabrikerede komponenter, er optisk fælde loading vist i <stron...

Watch this Scientific Journal Video about Optical Trap Loading of Dielectric Microparticles In Air at JoVE.com

Optical Trap Loading of Dielectric Microparticles In Air

A protocol for launching and stably trapping selected dielectric microparticles in air is presented.

Optisk Trap Loading af Dielektrisk mikropartikler i Air

We demonstrate a method to trap a selected dielectric microparticle in air using radiation pressure from a single-beam gradient optical trap. Randomly ...

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The Preparation of Electrohydrodynamic Bridges from Polar Dielectric Liquids

Fremstillingen af ​​elektrohydrodynamisk Bridges fra Polar Dielektriske Væsker

Horizontal and vertical liquid bridges are simple and powerful tools for exploring the interaction of high intensity electric fields (8-20 kV/cm) and ...

Writing Bragg Gratings in Multicore Fibers

Skrivning Bragg Riste i Multicore Fibers

Fiber Bragg gratings in multicore fibers can be used as compact and robust filters in astronomical and other research and commercial applications. Strong ...

Fabrication Process of Silicone-based Dielectric Elastomer Actuators

Fabrication Processen med silikone-baserede Dielektriske Elastomer aktuatorer

This contribution demonstrates the fabrication process of dielectric elastomer transducers (DETs). DETs are stretchable capacitors consisting of an ...

Measurement of Quantum Interference in a Silicon Ring Resonator Photon Source

Måling af Quantum Interference i en Silicon Ring resonator Photon Source

Silicon photonic chips have the potential to realize complex integrated quantum information processing circuits, including photon sources, qubit ...

Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications

Fremstilling af polymere mikrosfærer til optisk resonator og laser applikationer

This paper describes three methods of preparing fluorescent microspheres comprising &#960;-conjugated or non-conjugated polymers: vapor diffusion, ...

Application of Design Aspects in Uniaxial Loading Machine Development

Anvendelse af designaspekter i enakset lastning maskine udvikling

In terms of accurate and precise mechanical testing, machines run the continuum. Whereas commercial platforms offer excellent accuracy, they can be ...