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DOI :
10.3791/54863-v
November 29th, 2016
Chapters
0:05
Title
0:32
Preparation of Gel, Infrared Fluorescent Dye-labeled Probes and Unlabeled Probes
4:49
Binding Reaction and Electrophoresis
6:37
Imaging
7:35
Results: Electrophoretic Mobility Shift Assays Using Purified Proteins and Dye-labeled DNA Probes
8:57
Conclusion
우리는 여기서 중요한 생물학적 문제를 해결하기 위해 사례 연구와 같은 적외선 형광 염료 표지 DNA 프로브와 함께 정제 된 SOX-2 단백질을 사용하여 형광 전기 영동 이동성 이동 분석 (fEMSA)의 최적화 된 프로토콜을 설명합니다.
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