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DOI :
10.3791/54874-v
May 5th, 2017
Chapters
0:00
Title
1:38
Preparation of Formvar Grid Film
3:53
Preparation of Freeze-substitution Medium
5:06
Preparation of Cells
6:11
Liquefaction of Propane
7:32
Plunge Freezing of Samples
8:32
Freeze-substitution
9:04
Sample Warming
10:57
Results: Plunge Freezing Results in Improved Preservation of the Intracellular Structures
12:50
Conclusion
この原稿は、透過型電子顕微鏡により懸濁細胞を可視化するために使いやすく、低コストの低温固定の方法を記載しています。
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