Name: laser-induced chronic ocular hypertension model on sd rats
Uploaded: 2007-12-04T04:20:00
Description: Watch this Scientific Journal Video about Laser-Induced Chronic Ocular Hypertension Model on SD Rats at JoVE.com

<p class="jove_title"> Controllare e preparare le attrezzature </p><ol><li> Anestetizzare il ratto di iniezione intra-peritoneale di ketamina (80mg/kg) e xilazina (8mg/kg) (rapporto tra volume a 2:1). </li><li> Applicare una goccia di 0,5% Alcaine agli occhi ratto come anestetici topici prima fotocoagulazione laser. </li><li> Posizionare il ratto ed esporre le vene bersaglio con una pinzetta curva. Utilizzare il passo per iniziare fotocoagulazione laser. Applicare attacco laser 60 posti attorno alla vena limbari (eccetto l&#39;area nasale) e sp...

<ol>
	<li>Chan, H. -. C., Chang, R. C. C., Koon-Ching Ip, A., Chiu, K., Yuen, W. -. H., Zee, S. -. Y., So, K. -. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuroprotective+effects+of+Lycium+barbarum+Lynn+on+protecting+retinal+ganglion+cells+in+an+ocular+hypertension+model+of+glaucoma.">Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma.</a> <em style='font-style: italic'>Experimental Neurology</em>. <b>203</b>, 269-273 (2007).</li><li>Ji, J. -. Z., Elyaman, W., Yip, H. K., Lee, V. W. H., Yick, L. -. W., Hugon, J., So, K. -. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=CNTF+promotes+survival+of+retinal+ganglion+cells+after+induction+of+ocular+hypertension+in+rats:+the+possible+involvement+of+STAT3+pathway.">CNTF promotes survival of retinal ganglion cells after induction of ocular hypertension in rats: the possible involvement of STAT3 pathway.</a> <em style='font-style: italic'>European Journal of Neuroscience</em>. <b>19</b>, 265-272 (2004).</li><li>Li, R. S., Chen, B. -. Y., Tay, D. K., Chan, H. H. L., Pu, M. -. L., So, K. -. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Melanopsin-Expressing+Retinal+Ganglion+Cells+Are+More+Injury-Resistant+in+a+Chronic+Ocular+Hypertension+Model.">Melanopsin-Expressing Retinal Ganglion Cells Are More Injury-Resistant in a Chronic Ocular Hypertension Model.</a> <em style='font-style: italic'>Investigative Ophthalmology &amp; Visual Science</em>. <b>47</b>, 2951-2958 (2006).</li><li>Schori, H., Kipnis, J., Yoles, E., WoldeMussie, E., Ruiz, G., Wheeler, L. A., Schwartz, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vaccination+for+protection+of+retinal+ganglion+cells+against+death+from+glutamate+cytotoxicity+and+ocular+hypertension:+Implications+for+glaucoma.">Vaccination for protection of retinal ganglion cells against death from glutamate cytotoxicity and ocular hypertension: Implications for glaucoma.</a> <em style='font-style: italic'>PNAS</em>. <b>98</b>, 3398-3403 (2001).</li><li>WoldeMussie, E., Ruiz, G., Wijono, M., Wheeler, L. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuro-protection+of+retinal+ganglion+cells+by+brimonidine+in+rats+with+laser-induced+chronic+ocular+hypertension.">Neuro-protection of retinal ganglion cells by brimonidine in rats with laser-induced chronic ocular hypertension.</a> <em style='font-style: italic'>Investigative Ophthalmology &amp; Visual Science</em>. <b>42</b>, 2849-2855 (2001).</li></ol>

<p> Dopo due trattamenti laser con 7 giorni di distanza, siamo in grado di elevare la pressione intraoculare dell&#39;occhio laser photocoagulated del 50% confrontare con gli occhi di controllo. L&#39;elevata IOP può mantenere per almeno per 3 mesi dopo il trattamento laser per primo. Questo modello di ratto della pressione intraoculare elevata offrire preziose opportunità di studiare i meccanismi di pressione La perdita delle cellule gangliari della retina e danno del nervo ottico che imitano il cambiamento patologico nell&#39;uomo glaucoma. </p>

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<td>ULTIMA&#174; 2000SE Argon Laser </td>
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<td>Coherent Inc.</td>
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<td>Protective goggles</td>
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laser-induced chronic ocular hypertension model on sd rats

<p class="jove_content">Il glaucoma è una delle principali cause di cecità nel mondo. Pressione intraoculare elevata è un importante fattore di rischio. Fotocoagulazione laser ipertensione oculare indotta è uno dei modelli animali ben stabilita. Questo video mostra come indurre ipertensione oculare fotocoagulazione con Argon laser nel ratto.</p>

Watch this Scientific Journal Video about Laser-Induced Chronic Ocular Hypertension Model on SD Rats at JoVE.com

Laser-Induced Chronic Ocular Hypertension Model on SD Rats

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