Name: laser-induced chronic ocular hypertension model on sd rats
Uploaded: 2007-12-04T04:20:00
Description: Watch this Scientific Journal Video about Laser-Induced Chronic Ocular Hypertension Model on SD Rats at JoVE.com

<p class="jove_title">機器を確認し、準備する</p><ol><li>ケタミン（80mg/kg）とキシラジン（8mg/kg）（2:1体積比）の腹腔内注射によってラットをAnaesthetize。 </li><li>レーザー光凝固術前に局所麻酔薬としてラットの眼に0.5％alcaineの一滴を適用します。 </li><li>ラットを配置し、湾曲鉗子を使用してターゲットの静脈を公開。レーザー光凝固を開始するために足音を使用してください。角膜輪部静脈（鼻のエリアを除く）?...

<ol>
	<li>Chan, H. -. C., Chang, R. C. C., Koon-Ching Ip, A., Chiu, K., Yuen, W. -. H., Zee, S. -. Y., So, K. -. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuroprotective+effects+of+Lycium+barbarum+Lynn+on+protecting+retinal+ganglion+cells+in+an+ocular+hypertension+model+of+glaucoma.">Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma.</a> <em style='font-style: italic'>Experimental Neurology</em>. <b>203</b>, 269-273 (2007).</li><li>Ji, J. -. Z., Elyaman, W., Yip, H. K., Lee, V. W. H., Yick, L. -. W., Hugon, J., So, K. -. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=CNTF+promotes+survival+of+retinal+ganglion+cells+after+induction+of+ocular+hypertension+in+rats:+the+possible+involvement+of+STAT3+pathway.">CNTF promotes survival of retinal ganglion cells after induction of ocular hypertension in rats: the possible involvement of STAT3 pathway.</a> <em style='font-style: italic'>European Journal of Neuroscience</em>. <b>19</b>, 265-272 (2004).</li><li>Li, R. S., Chen, B. -. Y., Tay, D. K., Chan, H. H. L., Pu, M. -. L., So, K. -. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Melanopsin-Expressing+Retinal+Ganglion+Cells+Are+More+Injury-Resistant+in+a+Chronic+Ocular+Hypertension+Model.">Melanopsin-Expressing Retinal Ganglion Cells Are More Injury-Resistant in a Chronic Ocular Hypertension Model.</a> <em style='font-style: italic'>Investigative Ophthalmology &amp; Visual Science</em>. <b>47</b>, 2951-2958 (2006).</li><li>Schori, H., Kipnis, J., Yoles, E., WoldeMussie, E., Ruiz, G., Wheeler, L. A., Schwartz, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vaccination+for+protection+of+retinal+ganglion+cells+against+death+from+glutamate+cytotoxicity+and+ocular+hypertension:+Implications+for+glaucoma.">Vaccination for protection of retinal ganglion cells against death from glutamate cytotoxicity and ocular hypertension: Implications for glaucoma.</a> <em style='font-style: italic'>PNAS</em>. <b>98</b>, 3398-3403 (2001).</li><li>WoldeMussie, E., Ruiz, G., Wijono, M., Wheeler, L. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuro-protection+of+retinal+ganglion+cells+by+brimonidine+in+rats+with+laser-induced+chronic+ocular+hypertension.">Neuro-protection of retinal ganglion cells by brimonidine in rats with laser-induced chronic ocular hypertension.</a> <em style='font-style: italic'>Investigative Ophthalmology &amp; Visual Science</em>. <b>42</b>, 2849-2855 (2001).</li></ol>

<p>離れて7日間二つのレーザー治療の後、我々はコントロールの目と比較して50％レーザーphotocoagulated眼のIOPを昇格させることができます。高架IOPは最初のレーザー治療後少なくとも3ヶ月間のために維持することができます。高眼圧のこのラットモデルはヒトの緑内障の病理学的変化を模倣する圧力誘起網膜神経節細胞の消失および視神経の損傷のメカニズムを研究する貴重な機会を提供しています。 </p>

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<td>ULTIMA&#174; 2000SE Argon Laser </td>
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<td>Protective goggles</td>
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laser-induced chronic ocular hypertension model on sd rats

<p class="jove_content">緑内障は、世界の失明の主要原因の一つです。高眼圧は、主要な危険因子です。レーザー光凝固誘発高眼圧症は十分に確立された動物モデルの一つです。このビデオでは、ラットでのアルゴンレーザー光凝固術によって高眼圧症を誘発する方法を示します。</p>

Watch this Scientific Journal Video about Laser-Induced Chronic Ocular Hypertension Model on SD Rats at JoVE.com

Laser-Induced Chronic Ocular Hypertension Model on SD Rats

SDラットにおけるレーザー誘起慢性高眼圧症モデル

Glaucoma is one of the major causes of blindness in the world. Elevated intraocular pressure is a major risk factor. Laser photocoagulation induced ocular ...

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