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DOI :
10.3791/54901-v
•
8:40 min
November 29th, 2016
Chapters
0:05
Title
0:35
Dissection of Adult Worms for Obtaining Gonads
2:21
Gonad Fixation
4:22
Blocking and Antibody-staining
6:18
Mounting the Tissue
7:15
Results: ERK Activity in Germline Tissues
7:53
Conclusion
我们目前在解剖C.积极ERK的时间和空间定位的免疫基于成像的方法线虫性腺。这里所描述的协议可以适于任何信令的可视化或结构蛋白中的C.线虫性腺,提供了合适的抗体试剂是可用的。
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