原子力LINCコンプレックス全体に機械的な力を測定するために、蛍光共鳴エネルギー移動（FRET）-forceのバイオセンサーを使用するためのプロトコル

A number of FRET-based force biosensors have recently been developed, enabling the protein-specific resolution of intracellular force. In this protocol, we demonstrate how one of these sensors, designed for the linker of the nucleoskeleton-cytoskeleton (LINC) complex protein Nesprin-2G can be used to measure actomyosin forces on the nuclear LINC complex.