Immunofluorescence and Linear Invadosome Quantification
Transcript
Our group works on the interaction between cell and extracellular matrix. Cells can produce but also can degrade the extracellular matrix element. Degradation of the matrix can occur during physiological but also in pathological processes, such as
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Denne protokol beskriver, hvordan man forbereder en 2D-blandet matrix, der består af gelatine og kollagen I, og et 3D-collagen-I-stik for at studere lineære invadosomer. Disse protokoller tillader undersøgelsen af lineær invadosomdannelse, matrixnedbrydningsaktivitet og invasionskapaciteterne af primære celler og cancercellelinier.