January 13th, 2017
•Here we present a detailed protocol of (A) the identification of a natural product with antibiotic activity, (B) the purification of the compound, (C) the first model of its biosynthesis, (D) genome sequencing/-mining and the (E) verification of the biosynthetic gene cluster.
Tags
Related Videos
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved