Name: 2-methacryloyloxyethyl phosphorylcholine polymer treatment of complete dentures to inhibit denture plaque deposition
Uploaded: 2016-12-26T15:00:00
Description: Watch this Scientific Journal Video about 2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition at JoVE.com

作者要感谢所有的参与者，牙医和助手为他们参与这项研究。我们衷心感谢广隆桑田教授和助理伊藤博文森崎教授，口腔微生物学系和免疫学，牙科昭和大学，他们的支持。

<html> 1. PMBPAz溶液的制备（图1） <ol><li>合成MPC继先前报告的工业过程15。 </li><li>使用在乙醇MPC，BMA，和MPAz的常规自由基聚合合成光反应的MPC聚合物PMBPAz，如前所述14。 注：PMBPAz应含有上述单体单元的60％，30％和10％（摩尔％），分别为。冷藏PMBPAz溶解在乙醇中以制备在使用前用0.5％（重量）溶液。 </li><li>处理的PMMA表面之前，允许PMBPAz乙醇溶液放置在室温下30分钟。 </li></ol><...

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在PMBPAz涂料机制它是具有挑战性的结合的MPC聚合物为聚甲基丙烯酸甲酯表面以简单和稳定的方式。这里，我们使用一个新的光反应性单体轴承phenylazide基，MPAz，它通过常规的自由基聚合过程与其它单体聚合，得到具有phenylazide侧链的聚合物。的phenylazide组由紫外线照射分解形成氮宾基团，其是可以共价结合到烷基高度反应性的自由基团。因此，含有MPAz单元基于MPC-聚合物可结合于...

口腔护理对预防在体弱的老人机会性感染和吸入性肺炎的重要作用。口腔护理，包括每餐后刷牙，清洗假牙，和接收专业口腔保健，在减少此类疾病1-3的发病率的一个重要的角色。尤其是口腔卫生不良强烈吸入性肺炎在体弱的老人有关。因此，更应重视对口腔健康护理老年病人，谁都有自己4清洁假牙自己的能力有限。 即使在发达国家，采用亚克力，树脂类义齿的仍然是无牙老年患者5最常见的治疗选择。鉴于它们的高吸水能力，微孔表面，和疏水性质，丙烯酸义齿基托从聚甲基丙烯酸甲酯构成（PMMA）容易积聚在其表面，这就是所谓的假牙牌匾上牙菌斑。 2-甲基丙烯酰磷酸胆碱（MPC）聚合物是公知的生物聚合物材料6具有因为它们对蛋白质的吸附和细胞粘附7-11高电阻的显着的抗血栓和组织相容性。 MPC聚合物涂层已经在各种医疗设备被应用，以防止感染12和已建议有防止对聚甲基丙烯酸甲酯义齿表面13义齿菌斑积累的潜力。在临床应用中的MPC聚合物的聚甲基丙烯酸甲酯假牙的挑战是要可靠地将它们绑定到聚甲基丙烯酸甲酯以简单和稳定的方式面。先前，报道的MPC聚合物通过接枝技术可以稳定地结合到聚甲基丙烯酸甲酯的表面和具有抑制牙斑积聚的可能性。然而，接枝技术并不简单，需要专门的设备，WHICH使得其临床应用的挑战。这里，我们描述了利用光反应性磷脂的聚合物，具体地说，就是光反应性甲基丙烯酸酯衍生物，2-甲基丙烯酰-4- azidobenzoate（MPAz）-synthesized完成各种不同类型的材料的表面修饰的一个新的MPC涂层过程。 MPAz是共聚合与MPC聚合物和甲基丙烯酸正丁酯（BMA），得到聚（MPC- 共同 -BMA- 共 -MPAz）（PMBPAz）。 PMBPAz可通过紫外（UV）光照射14下MPAz所述叠氮基团的活化共价结合到聚甲基丙烯酸甲酯假牙的表面上。该过程不需要任何专门的设备，并且可以通过临床医生2分钟内完成。我们还在临床应用这个过程，并展示了其临床效用和功效在义齿抑制斑块沉积。

<table border="0" cellpadding="0" cellspacing="0" width="695">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:236px;">Ultrasonic Cleaner</td>
			<td style="width:104px;">Aiwa Medical Engineering</td>
			<td style="width:116px;">AU-12C</td>
			<td style="width:239px;">Clean in distilled water before stain the denture</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">UVP UV Crosslinker DL-1000</td>
			<td>Funakoshi</td>
			<td>95-0174-03</td>
			<td>UV light irradiation for 2min before use</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Lightbox S</td>
			<td>Suntec</td>
			<td align="right">6542</td>
			<td>capturing in this box after stain the denture</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Adobe Photoshop CS6 Extended</td>
			<td>Adobe</td>
			<td></td>
			<td>Calucurate and quantify</td>
		</tr>
	</tbody>
</table>

2-methacryloyloxyethyl phosphorylcholine polymer treatment of complete dentures to inhibit denture plaque deposition

Removable dentures made of poly (methyl methacrylate) (PMMA) are prone to bacterial adherence and dental plaque formation, which is called denture plaque. Denture plaque-associated infection is a source of serious dental and medical complications in the elderly. 2-Methacryloyloxyethyl phosphorylcholine (MPC) is a well-known biomedical material that exhibits marked antithrombogenicity and tissue compatibility because of its high resistance to protein adsorption and cell adhesion. Therefore, MPC polymer coatings are suggested to have the potential to inhibit plaque deposition on the surface of PMMA dentures. However, coating MPC polymer on the surface of a PMMA denture is a complex procedure that requires specialized equipment, which is regarded as a major barrier to its clinical application. 
Here, we introduce a new MPC polymer treatment procedure that uses poly (MPC-co-BMA-co-MPAz) (PMBPAz) to prevent denture plaque deposition on removable dentures. This procedure enables the MPC coating of PMMA denture surfaces in a simple and stable manner that is resistant to various chemical and mechanical stresses due to the MPC layer of PMBPAz that is covalently bound to the PMMA surface by ultraviolet light irradiation. In addition, the procedure does not require any specialized equipment and can be completed by clinicians within 2 min. We applied this procedure in a clinical setting and demonstrated its clinical utility and efficacy in inhibiting plaque deposition on removable dentures.

PMBPAz处理使PMMA的MPC涂层表面通过简单的和短期的方法并维持MPC聚合物的菌斑抑制作用。此过程不需要任何专门的设备，并且可以通过临床医生2分钟内进行椅侧。以下协议步骤2中，PMBPAz治疗11例无牙颌可拆卸全口义齿在临床上成功地进行，没有任何实质性的问题。控制假牙的粘膜和抛光的表面上的平均百分比菌斑指数分别为40.7％±19.9％和28.0％±16.8％，而那些PMBPAz处理的?...

Watch this Scientific Journal Video about 2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition at JoVE.com

2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition

Removable poly(methyl methacrylate) (PMMA) dentures are prone to bacterial adherence and plaque formation. Denture plaque-associated infection is a source of serious dental and medical complications in the elderly. This paper introduces a novel protocol to treat PMMA dentures with 2-methacryloyloxyethyl phosphorylcholine polymer, poly(MPC-co-BMA-co-MPAz), to suppress plaque deposition on PMMA dentures.

全口义齿的2-甲基丙烯酰磷酰胆碱聚合物处理，以抑制义齿斑块沉积

Removable dentures made of poly (methyl methacrylate) (PMMA) are prone to bacterial adherence and dental plaque formation, which is called denture plaque. ...

Research

JoVE Journal

Medicine

In vivo Near Infrared Fluorescence (NIRF) Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis

In vivo Near Infrared Fluorescence NIRF Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis

体内近红外荧光（NIRF）血管内分子成像炎性斑块，多式联运方针成像动脉粥样硬化

The vascular response to injury is a well-orchestrated inflammatory response triggered by the accumulation of macrophages within the vessel wall leading ...

科研

医学

Mouse Complete Stasis Model of Inferior Vena Cava Thrombosis

鼠标完成血瘀模型的下腔静脉血栓

Venous thromboembolism (VTE) includes both deep vein thrombosis (DVT) and pulmonary embolism (PE).  In the United States (U.S.), the high morbidity and ...

The Measurement and Treatment of Suppression in Amblyopia

测量和制止在弱视治疗

Amblyopia, a developmental disorder of the visual cortex, is one of the leading causes of visual dysfunction in the working age population. Current ...

The Goeckerman Regimen for the Treatment of Moderate to Severe Psoriasis

Goeckerman方案用于治疗中度至重度牛皮癣

Psoriasis is a  chronic, immune-mediated inflammatory skin disease affecting approximately 2-3%  of the population. The Goeckerman regimen consists of ...

A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology

A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology

一个人力<em&gt;前体内</em&gt;动脉粥样硬化斑块模型研究病变的生物学

Atherosclerosis is a chronic inflammatory disease of the vasculature. There are various methods to study the inflammatory compound in atherosclerotic ...

Network Analysis of Foramen Ovale Electrode Recordings in Drug-resistant Temporal Lobe Epilepsy Patients

在耐药性颞叶癫痫患者卵圆孔电极记录的网络分析

Approximately 30% of epilepsy patients are refractory to antiepileptic drugs. In these cases, surgery is the only alternative to eliminate/control ...

Complete and Partial Aortic Occlusion for the Treatment of Hemorrhagic Shock in Swine

完全性和部分主动脉闭塞治疗猪失血性休克

Hemorrhage remains the leading cause of preventable deaths in trauma. Endovascular management of non-compressible torso hemorrhage has been at the ...

Predicting Treatment Response to Image-Guided Therapies Using Machine Learning: An Example for Trans-Arterial Treatment of Hepatocellular Carcinoma

用机器学习预测图像引导疗法的治疗反应--肝细胞癌的跨动脉治疗实例

Intra-arterial therapies are the standard of care for patients with hepatocellular carcinoma who cannot undergo surgical resection. The objective of this ...

Direct Intrabronchial Administration to Improve the Selective Agent Deposition Within the Mouse Lung

直接注射内管理,改善小鼠肺内的选择性代理沉积

Intratracheal (IT) administration of experimental agents is an essential technique in murine models of diffuse lung diseases, such as bleomycin-induced ...

Establishment of a Severe Dry Eye Model Using Complete Dacryoadenectomy in Rabbits

在兔子中使用完全的白眼切除术建立严重干眼模型

Dry eye disease (DED) is a complex disease with multiple etiologies and variable symptoms, having ocular surface inflammation as its key pathophysiologic ...