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DOI :
10.3791/54967-v
April 26th, 2017
Chapters
0:05
Title
0:51
Construction of the PUF RNA-binding Domain Scaffold with Customized RNA Binding Specificity by Overlapping PCR
2:05
Transfection of Engineered Splicing Factors (ESFs) for Modulation of Exon Inclusion and Alternative Splice-site Use
6:24
Modulation of Endogenous Bcl-x Splicing and Apoptosis by ESFs
8:23
Results: Subcellular Localization of ESFs in Transfected HeLa Cells
9:30
Conclusion
3:43
Cell Harvest and Identification of Spliced Isoforms
このレポートは、具体的には、哺乳動物細胞において標的遺伝子のスプライシングを調節する新規な人工スプライシング因子(ASFS)を設計および構築する生物工学の方法を記載しています。この方法はさらに、RNA代謝の他の側面を操作するための様々な人工的な要因を設計するために拡張することができます。
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