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DOI :
10.3791/54981-v
February 10th, 2017
Chapters
0:05
Title
0:59
Isolation of Embryonic Midbrain
2:55
Dissociation and Plating of Cells
5:34
Harvesting Cultured Dopaminergic Neurons for RNA Sequencing
7:04
Generation, Purification and Amplification of First-strand cDNA
9:13
Results: RNA-seq Detection of Expressed Transcripts in Single Dopaminergic Neurons
9:39
Conclusion
帕金森病(PD),黑质(SNC)多巴胺能神经元退化,导致运动功能障碍。此处我们报告从表达eGFP的小鼠通过酪氨酸羟化酶(TH)的启动子序列驱动的培养腹侧中脑神经元,从培养收获个别荧光神经元,并使用RNA-SEQ测量它们转录的协议。
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