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DOI :
10.3791/54982-v
December 28th, 2016
Chapters
0:05
Title
3:46
Embedding the Condyle and Hind Leg for Frozen Sectioning
5:51
Slide Preparation and Basic Counterstaining
6:29
Immunohistochemical Staining for Runx2 and DMP1
8:04
Results: Cell Lineage Tracing
9:58
Conclusion
1:12
Activation of Cre and Whole Body Fixation
我们制定了两套在ROSA26 tdtomato(在所有细胞中广泛表达)/ Cre重组(特别是软骨细胞表达)小鼠跟踪组合:一个2.3Col1a1-GFP(具体以成骨细胞)和一个与免疫(具体以骨细胞)。该数据证明软骨细胞的直接转化成骨细胞。
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