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DOI :
10.3791/54982-v
December 28th, 2016
Chapters
0:05
Title
3:46
Embedding the Condyle and Hind Leg for Frozen Sectioning
5:51
Slide Preparation and Basic Counterstaining
6:29
Immunohistochemical Staining for Runx2 and DMP1
8:04
Results: Cell Lineage Tracing
9:58
Conclusion
1:12
Activation of Cre and Whole Body Fixation
2.3Col1a1-GFP(骨芽細胞に特異的)で1と(骨細胞に特異的)免疫蛍光との1:私たちは(特に軟骨細胞で発現さ)/のCreマウス(遍在すべての細胞で発現)Rosa26遺伝子tdtomatoのトレースの組み合わせの2セットを開発しました。データは、骨細胞への軟骨細胞の直接形質転換を実証します。
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