Name: an experimental and finite element protocol to investigate the transport of neutral and charged solutes across articular cartilage
Uploaded: 2017-04-23T13:00:00
Description: Watch this Scientific Journal Video about An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage at JoVE.com

Forfatterne ønsker å uttrykke sin takknemlighet til Mr. Jeroen van den Berg og Mr. Matthijs Wassink fra utviklings mekanikk gruppen ved UMC Utrecht for deres hjelp i pakkeprosessen av osteochondral plugger. Dette arbeidet ble støttet av et stipend fra nederlandsk Arthritis Foundation.

MERK: Protokollen som presenteres her er adoptert fra de eksperimentelle og beregningsprosedyrer av nyere forskningsrapporter 6, 8, 9. Protokollen er illustrert i figur 1. De avdød materialene ble samlet inn med tillatelse fra veterinær fakultet Utrecht University. 1. Prøve og Bath Preparat <ol><li> Bore ut sylindriske osteokondrale plu...

<ol>
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A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transport+of+neutral+solute+across+articular+cartilage+and+subchondral+plate.">Transport of neutral solute across articular cartilage and subchondral plate.</a> , (2016).</li><li>Pouran, B., Arbabi, V., Weinans, H., Zadpoor, A. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+multiphysics+models+to+efficient+design+of+experiments+of+solute+transport+across+articular+cartilage.">Application of multiphysics models to efficient design of experiments of solute transport across articular cartilage.</a> Comput Biol Med. 78, 91-96 (2016).</li><li>Hosseini, S. M., Wu, Y., Ito, K., Donkelaar, C. 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G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diffusion-tensor+imaging+of+human+articular+cartilage+specimens+with+early+signs+of+cartilage+damage.">Diffusion-tensor imaging of human articular cartilage specimens with early signs of cartilage damage.</a> Radiology. 266 (3), 831-841 (2013).</li><li>Tavakoli Nia, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aggrecan+Nanoscale+Solid-Fluid+Interactions+Are+a+Primary+Determinant+of+Cartilage+Dynamic+Mechanical+Properties.">Aggrecan Nanoscale Solid-Fluid Interactions Are a Primary Determinant of Cartilage Dynamic Mechanical Properties.</a> ACS Nano. 9 (3), 2614-2625 (2015).</li><li>Arbabi, V., Campoli, G., Weinans, H., Zadpoor, A. 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Vi presentert en eksperimentell protokoll kombinert med en endelig elementmodellering fremgangsmåte for å studere diffusjon av nøytrale og ladede oppløste stoffer på tvers av leddbrusk. Ifølge våre nyere studier, kan de foreslåtte modellene nøyaktig beskrive transport av både nøytral (bifasisk-oppløst stoff) og negativt ladede (av multi) oppløsninger på tvers av ulike soner av leddbrusk 8, 9. Det er derfor antatt at leddbrusken blir funksjonsmessig...

<html> Molekyl transport spiller en vital rolle i homeostasen av artikulerte ledd, levering av terapeutiske midler til leddbrusk og kontrastforsterket avbildning brusk 1, 2, 3. Faktorer som brusk integrasjon og intakthet, oppløst stoff ladning og størrelse, så vel som osmolalitet og konsentrasjon av badet i kontakt med brusk kan påvirke transporthastigheten 4, 5, 6. Transporten av de oppløste stoffer, enten nøytrale eller ladede, kan være forskjellig mellom leddbrusk soner, fordi hver sone består av forskjellige konsentrasjoner og orienteringer av store ekstracellulære matriks-molekyler, nemlig proteoglykaner (PG-er) og kollagen type II-1, 7, 8, 9,lass = &quot;ekstern referanse&quot;&gt; 10, 11. Enda viktigere, kan transporten av ladede oppløste stoffer være sterkt avhengig av konsentrasjonen av proteoglykaner som omfatter negative faste ladninger i den ekstracellulære matriks som øker på tvers av leddbrusk 8, 9. Disse parametrene særlig fast ladningstetthet (FCD), kan orienteringen av kollagenfibriller og vanninnhold variasjon på tvers av brusk gjennomgå forandringer som osteoartritt (OA) skrider frem, og derved betegner viktigheten av å studere diffusjon over brusk. I denne studien, en protokoll basert på tidligere etablerte eksperimentelle studier og beregnings 6, 8, 9 er foreslått for nøyaktig å undersøke diffusjon under forskjellige grensebetingelser ved bruk av nøytrale og ladede stoffer i en endelig-bad modell av diffusjon. THan foreslo metoder er sammensatt av mikro-Computed Tomography bildebehandling (mikro-CT) til et system med brusk og en endelig-bad med avansert bifasisk-oppløst stoff og multifaset endelig-element-modeller. Disse modellene gjør det mulig å oppnå de diffusjonskoeffisientene nøytrale og ladede molekyler, så vel som FCDs på tvers av ulike soner av leddbrusk. Ved hjelp av disse modellene, kan man få bedre forståelse av oppførselen til Diffuseranordningen nøytrale og ladede molekyler som kan brukes til å undersøke samspillet mellom brusk og overliggende endelig-bad.

<table border="0" cellpadding="0" cellspacing="0" width="688">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Hexabrix</td>
			<td style="width:149px;">Guerbet</td>
			<td style="width:123px;">15HX005D</td>
			<td style="width:201px;">Negatively charged contrast agent</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Visipaque</td>
			<td style="width:149px;">GE healthcare</td>
			<td align="right" style="width:123px;">12570511</td>
			<td>Nuetral contrast agent</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">PBS</td>
			<td style="width:149px;">Life technologies</td>
			<td align="right" style="width:123px;">10010023</td>
			<td>Medium</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">micro-CT</td>
			<td style="width:149px;">Perkin Elmer</td>
			<td style="width:123px;"></td>
			<td>Monitoring diffusion</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Freezing-point osmometer</td>
			<td style="width:149px;">Advanced instruments</td>
			<td style="width:123px;"></td>
			<td>Measuring solution osmolality</td>
		</tr>
	</tbody>
</table>

an experimental and finite element protocol to investigate the transport of neutral and charged solutes across articular cartilage

<html> Osteoartritt (OA) er en ødeleggende sykdom som er assosiert med degenerering av leddbrusk og subkondrale ben. Degenerering av leddbrusk svekker dets lastbærende funksjon i det vesentlige som den opplever enorm kjemisk nedbrytning, det vil si proteoglykan og kollagen tap fibril avbrudd. En lovende måte å undersøke kjemiske skademekanismer i løpet av OA er å eksponere brusk prøvene til en ekstern oppløst stoff og overvåke diffusjon av molekyler. Graden av bruskskader (dvs. konsentrasjon og konfigurasjon av essensielle makromolekyler) er forbundet med kollisjonsenergi tap av ytre oppløselige forbindelser mens flytter over leddbrusk gir forskjellige diffusjonsegenskaper i forhold til frisk brusk. I denne studien, introduseres en protokoll, som består av flere trinn og er basert på tidligere utviklet eksperimentell mikro-C14 piksler; &quot;&gt; omputed T omography (mikro-CT) og endelig elementmodellering for transport av ladede og uladede joderte molekyler er først registrert ved hjelp av mikro-CT, som blir fulgt ved å anvende to-fase-oppløst stoff og multifaset endelig-element-modeller for å oppnå diffusjonskoeffisienter. og faste ladningstettheter på tvers av brusk soner.

De representative resultatene gitt her er adoptert fra tidligere forskningsrapporter 6, 8, 9, 16. I OA, gjennomgår leddbrusk vesentlige endringer viktigst GAG tap, og collagen fibril skade 17, 18, 19. Disse...

Watch this Scientific Journal Video about An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage at JoVE.com

An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage

Vi foreslår en protokoll for å undersøke transporten av ladede og uladede molekyler på tvers av leddbrusk ved hjelp av nylig utviklede eksperimentelle og numeriske metoder.

En eksperimentell og Finite Element-protokollen for å undersøke Transport av nøytrale og siktet Oppløste stoffer over Leddbrusk

Osteoarthritis (OA) is a debilitating disease that is associated with degeneration of articular cartilage and subchondral bone. Degeneration of articular ...

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