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DOI :
10.3791/54995-v
July 23rd, 2017
Chapters
0:00
Title
0:09
Introduction
1:03
Cannulation and Perfusion
3:55
Purification and Handpicking
6:30
Troubleshooting: Common Bile Duct Distended During Perfusion
7:03
Troubleshooting: Duodenum Distended During Perfusion
7:37
Islet Transplantation at EFP Site
10:23
Results
11:29
Conclusion
このプロトコルは、マウス島の単離および脱細胞化された骨格上への播種を実証する。足場支持膵島を、ストレプトゾトシン(STZ)誘発糖尿病マウスの精巣上体脂肪パッドに移植した。島は移植部位で生存し、高血糖状態を逆転させた。
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