Nuclear Magnetic Resonance spektroskopi for identifisering av flere Phosphorylations med egen Uordnede Proteiner

We describe here a method to identify multiple phosphorylations of an intrinsically disordered protein by Nuclear Magnetic Resonance Spectroscopy (NMR), using Tau protein as a case study. Recombinant Tau is isotopically enriched and modified in vitro by a kinase prior to data acquisition and analysis.