Lille University

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

Clément Danis^*1,2, Clément Despres^*1, Luiza M. Bessa¹, Idir Malki¹, Hamida Merzougui¹, Isabelle Huvent¹, Haoling Qi¹, Guy Lippens¹, François-Xavier Cantrelle¹, Robert Schneider¹, Xavier Hanoulle¹, Caroline Smet-Nocca¹, Isabelle Landrieu¹

¹UMR8576, CNRS, Lille University, ²UMR-S1172, INSERM CNRS, Lille University

We describe here a method to identify multiple phosphorylations of an intrinsically disordered protein by Nuclear Magnetic Resonance Spectroscopy (NMR), using Tau protein as a case study. Recombinant Tau is isotopically enriched and modified in vitro by a kinase prior to data acquisition and analysis.