Name: adsc-sheet transplantation to prevent stricture after extended esophageal endoscopic submucosal dissection
Uploaded: 2017-02-10T13:00:00
Description: Watch this Scientific Journal Video about ADSC-sheet Transplantation to Prevent Stricture after Extended Esophageal Endoscopic Submucosal Dissection at JoVE.com

This study was supported and funded by the Avenir Foundation (Fondation de l'Avenir, 255 rue de Vaugirard, 75719 Paris cedex 15, Paris, France). This study would never have been conducted without the precious help of the veterinary team of the Laboratory of Biosurgical Research from the Alain Carpentier Foundation.

Tutti gli animali sono stati trattati secondo il comitato etico Animal Research (linee guida del Ministero dell&#39;Agricoltura francese). Il protocollo ha ricevuto l&#39;approvazione del comitato etico locale autorizzato per sperimentazioni animali al Paris Descartes University (numero di registrazione MESR 2.035,02; Facoltà di Medicina Paris Descartes, Parigi, Francia). 1. ADSC cultura e etichettatura <ol><li> Ottenere ADSCs confermati da un istituto privato. Culture ADSCs allogeniche a 37 ° C e 5% di CO <...

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In questo modello di maiale, il trapianto di ADSC fogli era tecnicamente riuscita, e il vivo di valutazione PCle in permesso per il monitoraggio attecchimento delle cellule. valutazioni cliniche, endoscopici, radiologici e istologici hanno dimostrato l&#39;efficacia del foglio ADSC endoscopica alla prevenzione stenosi esofagea dopo ESD prolungato. Il trapianto endoscopica di una soluzione di glicerolo ADSC contenente un foglio di tintura indaco carminio è un approccio inno...

La gestione dei tumori esofagei superficiali è cambiato con lo sviluppo di nuove tecniche endoscopiche. Al giorno d&#39;oggi, la resezione endoscopica è il trattamento di prima linea. Infatti, è associata a tassi di morbilità e mortalità inferiori rispetto a un intervento chirurgico con risultati uguali oncologici 1, 2, 3. resezione endoscopica della mucosa (EMR) e la resezione endoscopica della sottomucosa (ESD) sono le tecniche più utilizzati. Nel caso di un tumore superficiale esteso, ESD è preferito. Rispetto a EMR, ESD permette resezione in blocco, indipendentemente dalle dimensioni della lesione e la forma 4, 5, 6. La principale complicazione ritardata di ESD è formazione di stenosi esofagea, che si verifica in genere tra una e due settimane dopo la resezione. studi pubblicati recenti hanno dimostrato che la formazione di stenosi è correlatodimensioni della resezione. Il giapponese endoscopica Society raccomanda di evitare ESD di dimensioni superiori a ¾ della circonferenza esofageo perché sono associati con lo sviluppo stenosi in più del 90% dei casi e sono responsabili di problemi di alimentazione severe e maggiore deterioramento della qualità della vita. La prevenzione della stenosi esofagea rimane difficile. Meccanismi coinvolti nella formazione di stenosi sono solo parzialmente noti. Stenosi formazioni sembra derivare dalla associazione di due diversi meccanismi: (1) pro-infiammatorie reclutamento cellulare e (2) un eccessivo sviluppo della fibrosi 7. Sono stati proposti diversi trattamenti preventivi. Tuttavia, i risultati non sono stati soddisfacenti, con pochi benefici e gli effetti collaterali gravi 8, 9. Recentemente, un team giapponese, Ohki et al. , Proposto trapiantare un strato di cellule monostrato di cellule della mucosa orale autologhe nella esophcicatrice ageal. Il trapianto è stato effettuato immediatamente dopo ESD 10, 11. Essi hanno dimostrato l&#39;efficacia di questo approccio innovativo, prima in un modello canino e poi nei pazienti. le cellule derivate dal tessuto adiposo stromali (ADSCs) sono promettenti nella medicina rigenerativa. La loro applicazione in diversi settori ha mostrato risultati interessanti, soprattutto nel processo di guarigione. Terapia ADSC offre diversi vantaggi, in quanto le cellule sono facilmente isolati e sono associati con proprietà anti-infiammatorie, effetti immunomodulanti locali, induzione neovascolarizzazione, e capacità di differenziazione in mesenchimali e linee non mesenchimali 12, 13, 14. In uno studio precedente, la nostra squadra ha dimostrato l&#39;efficacia del doppio trapianto endoscopica ADSC fogli per prev stenosi esofageaenzione dopo ESD prolungato in un modello di suini 15. In questo articolo, vengono presentati i rapporti di costruzione ADSC fogli e la tecnica di trapianto endoscopico.

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	<tbody>
		<tr height="20">
			<td height="20" style="height:20px;width:313px;">Transparent endoscopic cap Q180 compatible</td>
			<td style="width:159px;">Olympus Optical Co</td>
			<td style="width:107px;"></td>
			<td style="width:492px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">GIF-Q180 gastroscope&#160;</td>
			<td>Olympus Optical Co</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Videoscope System Exera II&#160;</td>
			<td>Olympus Optical Co</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Injection needle 18 G</td>
			<td>Olympus Optical Co</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Electrosurgery unit ERBE ICC 350&#160;</td>
			<td>ERBE Technology</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Indigo carmin 1%</td>
			<td>Life</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Endoscopic hybrid knife</td>
			<td>Life</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Minisonde Z pCLE green probe</td>
			<td>Mauna Kea Technology</td>
			<td></td>
			<td>You must learn how tu use the probe. The manipulation could be difficult</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fetal bovine serum</td>
			<td>Sigma Aldrich</td>
			<td>12105C</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Trypsin</td>
			<td>Sigma Aldrich</td>
			<td>T146</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Alpha minimum essential medium</td>
			<td>Thermo Fisher</td>
			<td>22561-021</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Phosphate-Buffered Salines</td>
			<td>Thermo Fisher</td>
			<td>10010-023</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">PKH67 dye kit</td>
			<td>Sigma Aldrich</td>
			<td>Mini67-KT</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">12-well temperature responsive cell culture dish</td>
			<td>Upcell Thermo Scientific</td>
			<td align="right">174900</td>
			<td>Feel the weel with 4 mL standard medium culture 30 minutes before seeding cells</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Esomeprazole 40 mg&#160;</td>
			<td>Biogaran</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Moprhine sulfate 50 mg/ mL</td>
			<td>Lavoisier</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Amoxicilline 1 g</td>
			<td>Biogaran</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Ketamine 250 mg/5 mL</td>
			<td>Panpharma</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Propofol 10 mg/ mL</td>
			<td>Fresenius</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Hydrophobic paper</td>
			<td>Carrefour</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

adsc-sheet transplantation to prevent stricture after extended esophageal endoscopic submucosal dissection

Negli anni passati, il costrutto cellule-foglio ha stimolato grande interesse nella medicina rigenerativa, in particolare per le procedure di chirurgia ricostruttiva. Lo sviluppo di tecnologie diversificate, che conciliano cellule stromali derivate dal tessuto adiposo (ADSCs) con vari biomateriali ha portato alla costruzione di numerosi tipi di sostituti tissutale, come le ossa, cartilagine e tessuto adiposo da roditore, suina o ADSCs umane. Esteso esofageo dissezione sottomucosa endoscopica (ESD) è responsabile della formazione di stenosi esofagea. prevenzione della stenosi rimane impegnativo, con trattamenti efficaci disponibili. Precedenti studi hanno riportato l&#39;efficacia del trapianto di cellule fogli mucosa in un modello canino e nell&#39;uomo. ADSCs vengono attribuite proprietà anti-infiammatorie, effetti di modulazione immunitaria locale, induzione neovascolarizzazione, e capacità di differenziazione in mesenchimali e lignaggi non mesenchimali. Questo studio originale descrive TRA endoscopicansplantation di un costrutto tissutale ADSC per evitare stenosi esofagea in un modello suina. Il costrutto ADSC era composto da due fogli ADSC allogeniche a strati l&#39;uno sull&#39;altro su una membrana di supporto cartaceo. Le ADSCs sono stati etichettati con il fluoroforo PKH67 per consentire sonda-based endomicroscopy laser confocale monitoraggio (PCle). Il giorno del trapianto, è stata effettuata una 5-cm e ESD emi-circonferenziale noti per indurre stenosi esofagea. Gli animali sono stati subito endoscopicamente trapiantati con 4 costrutti ADSC. L&#39;adesione completa dei costrutti ADSC stato ottenuto dopo 10 min di applicazione delicata. Gli animali sono stati sacrificati al giorno 28. Tutti gli animali sono stati trapiantati con successo. Il trapianto è stato confermato il giorno 3 con una valutazione PCle positivo. Rispetto agli animali trapiantati, gli animali di controllo hanno sviluppato gravi stenosi, con grande sviluppo del tessuto fibrotico, più frequenti problemi alimentari, e aumento di peso ridotto. Nel nostro modello, il trapianto di allogenic ADSCs, organizzati in fogli doppi cellulari, dopo ESD prolungato ha avuto successo e fortemente associata con un tasso di stenosi esofageo inferiore.

La cultura ADSCs e la procedura per ottenere il foglio ADSC è mostrato in Figura 1. La Figura 2 mostra la costruzione del trapianto, composto da due fogli ADSC livelli reciprocamente sulla loro membrana supporto cartaceo. ADSCs erano stati precedentemente etichettati con il fluoroforo PKH67 per consentire il monitoraggio del trapianto in vivo con PCle. La Figura 3 mostra le varie fasi estesa esofageo dissezione sottomu...

Watch this Scientific Journal Video about ADSC-sheet Transplantation to Prevent Stricture after Extended Esophageal Endoscopic Submucosal Dissection at JoVE.com

ADSC-sheet Transplantation to Prevent Stricture after Extended Esophageal Endoscopic Submucosal Dissection

Questo studio riporta un metodo efficace di endoscopico cellule stromali derivate dal tessuto adiposo (ADSC) -sheet trapianto per la prevenzione stenosi esofagea dopo una dissezione endoscopica della sottomucosa prolungato (ESD) in un modello suina.

ADSC fogli trapianto per prevenire stenosi dopo esteso esofagea endoscopica della sottomucosa dissezione

In past years, the cell-sheet construct has spurred wide interest in regenerative medicine, especially for reconstructive surgery procedures. The ...

Research

JoVE Journal

Medicine

Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using 18F-FDG PET

Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using 18F-FDG PET

Imaging non invasivo di acuta Alloinnesto rigetto dopo trapianto renale Rat Utilizzo<sup&gt; 18</sup&gt; F-FDG PET

The number of patients with end-stage renal  disease, and the number of kidney allograft recipients continuously increases.  Episodes of acute cellular ...

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Medicina

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