Name: adsc-sheet transplantation to prevent stricture after extended esophageal endoscopic submucosal dissection
Uploaded: 2017-02-10T13:00:00
Description: Watch this Scientific Journal Video about ADSC-sheet Transplantation to Prevent Stricture after Extended Esophageal Endoscopic Submucosal Dissection at JoVE.com

This study was supported and funded by the Avenir Foundation (Fondation de l'Avenir, 255 rue de Vaugirard, 75719 Paris cedex 15, Paris, France). This study would never have been conducted without the precious help of the veterinary team of the Laboratory of Biosurgical Research from the Alain Carpentier Foundation.

Bütün hayvanlar Hayvan Araştırmaları Etik Komitesi (Fransa Tarım Bakanlığı ve kurallar) göre tedavi edildi. protokol Paris Descartes Üniversitesi Hayvan Deneyleri yetkili yerel etik kurul onayı almış (kayıtlı numara Mesr 2.035,02; Tıp Fakültesi Paris Descartes, Paris, France). 1. ADSC Kültür ve Etiketleme <ol><li> Özel kurumdan teyit ADSCs edinin. 37 ° C&#39;de ve% 5,% 10 fetal dana serumu ve% 1 antibiyotik (penisilin ve streptomisin) dahil olmak üzere, a, minimum gerekli ortam maddesi i...

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Bu domuz modelinde, ADSC sayfalık nakli teknik olarak başarılı oldu ve in vivo PCIe değerlendirme hücre nakli izlenmesi için izin verdi. Klinik endoskopik, radyolojik ve histolojik değerlendirmeler genişletilmiş ESD sonrası özofagusdarlığı önleme endoskopik ADSC levha etkinliğini göstermiştir. Bir indigo karmin boya tabakasını içeren bir ADSC gliserol çözeltisi endoskopik nakli rejeneratif tıpta yenilikçi bir yaklaşımdır. Ohki ve ark. ilk endosk...

yüzeyel özofagus tümörlerinin yönetimi yeni endoskopik tekniklerin geliştirilmesi ile değişmiştir. Günümüzde, endoskopik rezeksiyon ilk basamak tedavi yöntemidir. Gerçekten de, eşit onkolojik sonuçlar 1, 2, 3 ile cerrahiye göre daha düşük morbidite ve mortalite oranları ile ilişkilidir. Endoskopik mukozal rezeksiyon (EMR) ve endoskopik submukozal rezeksiyon (ESD) en yaygın kullanılan yöntemlerdir. uzun bir yüzeysel tümör durumunda, ESD tercih edilir. EMR ile karşılaştırıldığında, ESD bağımsız lezyon büyüklüğü, bloğun rezeksiyon en izin verir ve 4, 5, 6, şekil. ESD ana gecikmiş komplikasyonu genellikle rezeksiyon sonrası bir ve iki hafta arasında gerçekleşir özofagusdarlığı oluşumdur. Son yayınlanan çalışmalar darlık oluşumu için korelasyon göstermiştirrezeksiyon boyutu. Japon Endoskopik Derneği ESD onlar olguların% 90&#39;ından fazlasında içinde darlık gelişimi ile ilişkili ve şiddetli beslenme sorunları ve yaşam kalitesinde önemli bozulma sorumlu çünkü özofagus çevresinin ¾ daha büyük boyutları kaçınarak önerir. Özofagus darlığı önlenmesi zor kalır. darlık oluşumunda rol oynayan mekanizmalar ancak kısmen bilinmektedir. (1) pro-enflamatuar hücre işe alma ve (2) aşırı fibrozis gelişimi 7: oluşumları darlık iki farklı mekanizma dernek kaynaklandığı görülmektedir. Çeşitli önleyici tedaviler önerilmiştir. Ancak sonuçlar çok az fayda ve ciddi yan etkilere 8, 9, tatmin edici idi. Son zamanlarda, bir Japon ekip, Ohki ve ark. , Esoph otolog oral mukozal hücrelerin, tek katmanlı hücre tabakasını nakli için önerilenageal yara. Transplantasyon ESD 10, 11 hemen sonra gerçekleştirildi. Önce bir köpek modelinde ve sonra hastalarda, bu yenilikçi yaklaşımın etkinliğini göstermiştir. Yağ dokusu kaynaklı stromal hücreler (ADSCs) rejeneratif tıpta umut vericidir. çeşitli alanlarda uygulama özellikle yara iyileştirici süreç içinde, ilginç sonuçlar göstermiştir. Hücreleri kolayca izole edilir ve mezenşimal olmayan mezenkimal soy, 12, 13, 14, anti-enflamatuar özellikleri, lokal immüno-modüle edici etkileri, yeni damar oluşumu indüksiyonu ve farklılaşma yetenekleri ile ilişkilidir, çünkü ADSC tedavi, pek çok avantajlar sunmaktadır. Daha önceki bir çalışmada, ekibimiz özofagusdarlığı prev için çift ADSC sayfalık endoskopik nakli etkinliğini göstermiştirBir domuz modelinde 15 genişletilmiş ESD sonrası ention. Bu makalede, ADSC sayfalık inşaat ve endoskopik nakli tekniği raporları sunulmuştur.

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	<tbody>
		<tr height="20">
			<td height="20" style="height:20px;width:313px;">Transparent endoscopic cap Q180 compatible</td>
			<td style="width:159px;">Olympus Optical Co</td>
			<td style="width:107px;"></td>
			<td style="width:492px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">GIF-Q180 gastroscope&#160;</td>
			<td>Olympus Optical Co</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Videoscope System Exera II&#160;</td>
			<td>Olympus Optical Co</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Injection needle 18 G</td>
			<td>Olympus Optical Co</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Electrosurgery unit ERBE ICC 350&#160;</td>
			<td>ERBE Technology</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Indigo carmin 1%</td>
			<td>Life</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Endoscopic hybrid knife</td>
			<td>Life</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Minisonde Z pCLE green probe</td>
			<td>Mauna Kea Technology</td>
			<td></td>
			<td>You must learn how tu use the probe. The manipulation could be difficult</td>
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			<td height="20" style="height:20px;">Fetal bovine serum</td>
			<td>Sigma Aldrich</td>
			<td>12105C</td>
			<td></td>
		</tr>
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			<td height="20" style="height:20px;">Trypsin</td>
			<td>Sigma Aldrich</td>
			<td>T146</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Alpha minimum essential medium</td>
			<td>Thermo Fisher</td>
			<td>22561-021</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Phosphate-Buffered Salines</td>
			<td>Thermo Fisher</td>
			<td>10010-023</td>
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			<td height="20" style="height:20px;">PKH67 dye kit</td>
			<td>Sigma Aldrich</td>
			<td>Mini67-KT</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">12-well temperature responsive cell culture dish</td>
			<td>Upcell Thermo Scientific</td>
			<td align="right">174900</td>
			<td>Feel the weel with 4 mL standard medium culture 30 minutes before seeding cells</td>
		</tr>
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			<td height="20" style="height:20px;">Esomeprazole 40 mg&#160;</td>
			<td>Biogaran</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Moprhine sulfate 50 mg/ mL</td>
			<td>Lavoisier</td>
			<td></td>
			<td></td>
		</tr>
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			<td height="20" style="height:20px;">Amoxicilline 1 g</td>
			<td>Biogaran</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Ketamine 250 mg/5 mL</td>
			<td>Panpharma</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Propofol 10 mg/ mL</td>
			<td>Fresenius</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Hydrophobic paper</td>
			<td>Carrefour</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

adsc-sheet transplantation to prevent stricture after extended esophageal endoscopic submucosal dissection

Geçmiş yıllarda, hücre sayfalık yapısı özellikle rekonstrüktif cerrahi prosedürleri için, rejeneratif tıpta geniş bir ilgi teşvik etmiştir. Çeşitli biyomateryallerle adipoz doku kaynaklı stromal hücreler (ADSCs) birleştiren çeşitli teknolojilerin geliştirilmesi gibi kemik, kıkırdak ve kemirgen, domuz veya insan ADSCs adipoz dokuların doku mühendisliği ikame, çeşitli türleri yapımı yol açmıştır. Genişletilmiş özofagus endoskopik submukozal diseksiyon (ESD) özofagusdarlığı oluşumu sorumludur. Darlık önleme hiçbir etkili tedaviler ile, zorlu kalır. Daha önce yapılan çalışmalar, bir köpek modelinde ve insanlarda mukozal hücre yapraklık nakli etkinliğini bildirilmiştir. ADSCs mezenşimal ve non-mezenkimal soy içine anti-inflamatuar özellikleri, lokal bağışıklık modülasyon efektleri, neovaskülarizasyon indüksiyon ve farklılaşma yeteneklerini ilişkilendirilir. Bu orijinal çalışma endoskopik tra açıklarBir ADSC doku mühendisliği yapının nsplantation bir domuz modelinde darlık önlemek için. ADSC yapısı bir kağıt desteği membran üzerinde birbirlerini katmanlı iki allojenik ADSC yaprak oluşmuştur. ADSCs prob bazlı konfokal lazer endomikroskopi (PCIe) izleme sağlamak için PKH67 florofor ile işaretlendi. transplantasyon gününde, 5-cm darlık indüklediği bilinen, yarı-dairesel ESD uygulandı. Hayvanlar hemen endoskopik 4 ADSC yapıları ile transplante edildi. ADSC yapılarının bir yapışma yumuşak uygulama, 10 dakika sonra elde edildi. Hayvanlar Tüm hayvanlar başarıyla transplante edildi 28. günde öldürüldü. Transplantasyon pozitif PCIe değerlendirme ile 3. günde teyit edilmiştir. nakledilen hayvanlara kıyasla, kontrol hayvanları ana fibrotik doku gelişimi, daha sık sindirim sorun ve indirgenmiş kilo alma ile, ağır striktür- geliştirdi. allogeni bizim modelinde, transplantasyonc çift hücre tabakalar halinde organize ADSCs, genişletilmiş ESD sonrası başarılı ve güçlü bir alt özofagus darlığı oranı ile ilişkili idi.

ADSCs ve ADSC tabaka elde etmek üzere işlemin kültürü, Şekil 1 &#39;de gösterilmiştir. Şekil 2 onların kağıt desteği membran üzerinde birbirlerini katmanlı iki ADSC yaprak oluşan greft yapımını gösterir. ADSCs önce PCIe in vivo aşı izleme sağlamak için PKH67 florofor ile işaretlendi. Şekil 3, 5 cm&#39;lik bir ve hemi-çevresel özofagus yara sonuçlanan uzun özofagus endoskopik submukozal dis...

Watch this Scientific Journal Video about ADSC-sheet Transplantation to Prevent Stricture after Extended Esophageal Endoscopic Submucosal Dissection at JoVE.com

ADSC-sheet Transplantation to Prevent Stricture after Extended Esophageal Endoscopic Submucosal Dissection

Bu çalışma endoskopik adipoz doku kaynaklı stromal hücre (ADSC) başarılı bir yöntem bildiren bir domuz modelinde uzun bir endoskopik submukozal diseksiyon (ESD) sonra özofagusdarlığı önlenmesi için -yaprak nakli.

ADSC sayfalık Transplantasyon Genişletilmiş Özofagus Endoskopik Submukozal Diseksiyon sonra darlık Önlemek için

In past years, the cell-sheet construct has spurred wide interest in regenerative medicine, especially for reconstructive surgery procedures. The ...

Research

JoVE Journal

Medicine

Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using 18F-FDG PET

Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using 18F-FDG PET

Sıçan Böbrek Nakli sonra Akut Allogreft Reddi non-invaziv Görüntüleme Kullanımı<sup&gt; 18</sup&gt; F-FDG PET

The number of patients with end-stage renal  disease, and the number of kidney allograft recipients continuously increases.  Episodes of acute cellular ...

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Hepatositlerin intrasplenic nakli düğümde kısmi Hepatectomy sonra. SCID fareler

Partial hepatectomy is a versatile and reproducible method to study liver regeneration and the effect of cell based therapeutics in various pathological ...

Discovering Middle Ear Anatomy by Transcanal Endoscopic Ear Surgery: A Dissection Manual

Transcanal endoskopik kulak cerrahi ile orta kulak anatomisi keşfetmek: diseksiyon el kitabı

The middle ear is located in the center of the temporal bone and bears a highly complex anatomy. The recently introduced exclusively endoscopic transcanal ...

Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model

Bir sıçan modelinde kısmi kolektomi sonra kaçağı azaltmak için Yağ dokusundan elde edilen kök hücre sayfasının nakli

Anastomotic leakage is a disastrous complication after colorectal surgery. Although current methods for leakage prevention have different levels of ...

Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

Ortotopik Karaciğer Transplantasyonunun Sıçan Modelinde Arteriyel Reconnection Sonrası Azaltılmış Komplikasyonlar

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation ...

Simultaneous Laryngopharyngeal and Conventional Esophageal pH Monitoring

Eşzamanlı Laringofaringeal ve Konvansiyonel Özofagus pH İzleme

In addition to typical reflux symptoms, many patients with gastroesophageal reflux disease (GERD) present with extraesophageal symptoms such as cough, ...

Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model

Murine Modelinde Miyokard İnfarktüsünden Sonra MSC Yüklemeli Enjekte Edilebilir Hidrojellerin intramiyokardiyal Transplantasyonu

One of the major issues facing current cardiac stem cell therapies for preventing postinfarct heart failure is the low retention and survival rates of ...

Digital Home-Monitoring of Patients after Kidney Transplantation: The MACCS Platform

Böbrek Nakli Sonrası Hastaların Dijital Evde İzlenmesi: MACCS Platformu

The MACCS (Medical Assistant for Chronic Care Service) platform enables secure sharing of key medical information between patients after kidney ...

Endoscopic Cholesteatoma Surgery

Endoskopik Kolesteatom Cerrahisi

Implementation of endoscopes in cholesteatoma surgery resulted in considerable changes in the management of cholesteatoma in the last two decades. ...

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model

Veno-Venöz Bypass Olmadan Domuz Karaciğeri Transplantasyonu Genişletilmiş Kriterli Donör Modeli Olarak

Liver transplantation is regarded as the gold standard for the treatment of a variety of fatal hepatic diseases. However, unsolved issues of chronic graft ...