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DOI :
10.3791/55019-v
•
10:16 min
January 6th, 2017
Chapters
0:05
Title
0:55
Western Blotting of CNTFRα Lysates
3:21
Immunocytochemistry in Combination with Lysosomal Inhibitors
6:00
Western Blot-detection of the pSTAT3 Levels
8:17
Results: Demonstration of CNTFRα-downregulation by Western Blotting, and Inhibition of Lysosomal Enzymes, and Immunocytochemistry
9:43
Conclusion
本协议描述印迹和免疫细胞化学以抑制溶酶体酶的组合如何西方可以用来演示睫状神经营养因子受体α(CNTFRα),它是由细胞因子样因子1(CLF-1之间的相互作用输送的下调)和内吞受体sorLA。
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