Name: quantification of strain in a porcine model of skin expansion using multi-view stereo and isogeometric kinematics
Uploaded: 2017-04-16T14:00:00
Description: Watch this Scientific Journal Video about Quantification of Strain in a Porcine Model of Skin Expansion Using Multi-View Stereo and Isogeometric Kinematics at JoVE.com

This work was supported by NIH grant 1R21EB021590-01A1 to Arun Gosain and Ellen Kuhl.

Denne protokol involverer dyreforsøg. Protokollen blev godkendt af IRB af Ann og Robert H. Lurie Børnehospital of Chicago Forskningscenter Animal Care og brug Udvalg at sikre human behandling af dyr. Resultaterne for to ekspansion undersøgelser under anvendelse af denne protokol er blevet offentliggjort andetsteds 16, 31. Udførelse af denne protokol kræver et team med supplerende ekspertise. Den første del af protokollen beskri...

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	<li>Gosain, A. K., Zochowski, C. G., Cortes, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Refinements+of+tissue+expansion+for+pediatric+forehead+reconstruction:+a+13-year+experience.">Refinements of tissue expansion for pediatric forehead reconstruction: a 13-year experience.</a> Plast Reconstr Surg. 124, 1559-1570 (2009).</li><li>Neumann, C. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+expansion+of+an+area+of+skin+by+progressive+distention+of+a+subcutaneous+balloon:+Use+of+the+Method+for+Securing+Skin+for+Subtotal+Reconstruction+of+the+Ear.">The expansion of an area of skin by progressive distention of a subcutaneous balloon: Use of the Method for Securing Skin for Subtotal Reconstruction of the Ear.</a> Plast Reconstr Surg. 19, 124-130 (1957).</li><li>De Filippo, R. 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<html> Her præsenterede vi en protokol til at karakterisere deformationerne induceret under en vævsekspansion procedure i en porcin model ved hjælp af multi-view stereo (MVS) og isogeometric kinematik (IGA kinematik). Under væv ekspansion, hud undergår store deformationer går fra en glat og forholdsvis flad overflade til en kuppel-lignende 3D form. Hud, ligesom andre biologiske membraner 34, reagerer at strække ved at producere nyt materiale, stigende i område, der kan anvendes derefter ti...

Tissue ekspansion er en almindelig teknik i plast og rekonstruktionskirurgi, der vokser hud in vivo for korrektionen af store kutane defekter 1. Neumann, i 1957, var den første kirurg til at dokumentere denne procedure. Han implanteret en ballon under huden af en patient og oppustet det gradvist over en periode på flere uger til at vokse nyt væv og dukke op igen et øre 2. Hud, ligesom de fleste biologiske væv, tilpasser sig påførte kræfter og deformationer for at nå mekanisk homøostase. Når den strækkes ud over den fysiologiske regime, hud vokser 3, 4. En af de centrale fordele ved vævsekspansion er produktion af huden med ordentlig vaskularisering og samme hår bærende, mekaniske egenskaber, farve og tekstur som det omgivende væv 5. Efter introduktionen seks årtier siden, hud udvidelse af pln er blevet bredt vedtaget af plast og rekonstruktive kirurger og anvendes for tiden til at korrigere forbrændinger, store medfødte defekter og for brystrekonstruktion efter mastektomi 6, 7. Men på trods af den udbredte anvendelse, kan huden udvidelse af procedurerne føre til komplikationer 8. Dette skyldes til dels manglen på tilstrækkelig kvantitativ dokumentation er nødvendig for at forstå den grundlæggende mechanobiology af proceduren og at vejlede kirurgen under præoperativ planlægning 9, 10. Vigtige parametre i denne teknik er opfyldningshastigheden, påfyldning volumen pr inflation, udvælgelse af formen og størrelsen af ekspanderen, og anbringelsen af anordningen 11, 12. Aktuel præoperativ planlægning afhænger i høj grad af lægens erfaring, hvilket resulterer i en bred vifte af vilkårlige protokoller, der ofte adskiller greatly 13, 14, 15. At løse de aktuelle videnhuller præsenterer vi en forsøgsprotokol at kvantificere ekspansion-induceret deformation i en porcin dyremodel af væv ekspansion. Protokollen bygger på anvendelsen af ​​multi-view stereo (MVS) at rekonstruere tredimensionelle (3D) geometrier ud af sekvenser af todimensionale (2D) billeder med ukendte kamerapositioner. Anvender noter, repræsentation af glatte overflader fører til beregningen af ​​de tilsvarende deformation kort ved hjælp af en isogeometric (IGA) beskrivelse. Analysen af geometrien er baseret på den teoretiske ramme af kontinuum mekanik af membraner med en udtrykkelig parametrisering 16. Karakterisering fysiologisk relevante deformationer af levende materialer over lange perioder stadig et udfordrende problem. Fælles strategier forbilleddannelse af biologiske væv indbefatter stereoskopisk digitalt billede korrelation, kommercielle motion capture systemer med reflekterende markører og biplan video fluoroskopi 17, 18, 19. Men disse teknikker kræver en restriktiv forsøgsopstilling, er generelt dyre, og primært blevet anvendt til ex vivo eller akut in vivo indstillinger. Hud har den fordel at være en tynd struktur. Selv om den består af flere lag, dermis er hovedansvarlige for de mekaniske egenskaber af vævet og således overfladen deformation er af primær betydning 20; rimelige kinematiske antagelser kan foretages med hensyn til ud af planet deformation 21, 22. Desuden er huden allerede udsat til det udendørs miljø, hvilket gør det muligt at bruge konventionelle billeddiagnostiske værktøjer til at fange sin geometri. Hførend vi foreslår anvendelsen af MVS som en overkommelig og fleksibel tilgang til at overvåge in vivo deformationer af huden over flere uger uden at interferere majorly med en vævsekspansion protokol. MVS er en teknik, som trækker 3D-gengivelser af genstande eller scener fra en samling af 2D-billeder med ukendt kameravinkler 23. Kun i de sidste tre år har flere kommercielle koder optrådte (se listen over materialer til eksempler). Den høje nøjagtighed af modellen genopbygning med MVS, med fejl så lavt som 2% 24, gør denne fremgangsmåde velegnet til den kinematiske karakterisering af hud in vivo over lange perioder. Til opnåelse af de tilsvarende deformation kort over huden under vævsekspansion, er punkterne mellem to vilkårlige geometriske udformninger matchet. Konventionelt har forskere i beregningsmæssige biomekanik anvendt finite element masker og omvendt analyse for at hente deformation kortet25, 26. IGA fremgangsmåde anvendes her anvender spline basisfunktioner som tilbyder flere fordele til analyse af tynde membraner 27, 28. Nemlig, tilgængeligheden af høje grad polynomier letter repræsentationer af glatte geometrier selv med meget grove masker 29, 30. Desuden er det muligt at montere den samme underliggende parametrering til alle overflade patches, der omgår behovet for et inverst problem at tage højde for ikke-matchende diskretisering. Den her beskrevne metode åbner nye muligheder for at studere hud mekanik i relevante in vivo indstillinger over lange perioder. Hertil kommer, vi håber, at vores metode er et gunstigt skridt mod det endelige mål at udvikle beregningsværktøjer for personlig behandling planlægning i det kliniske miljø. &lt;/ P&gt;

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			<td height="21" style="height:21px;width:216px;">Yucatan miniature swine</td>
			<td style="width:263px;">Sinclair Bioresources, Windham, ME</td>
			<td style="width:141px;">N/A</td>
			<td style="width:197px;"></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Antibiotics</td>
			<td>Santa Cruz Animal Health, Paso Robles, CA</td>
			<td style="width:141px;">sc-362931Rx</td>
			<td>Ceftiofur, dosage 5mg/kg intramuscular</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Chlorhexidine-based surgical soap</td>
			<td style="width:263px;">Cardinal Health, Dublin, OH</td>
			<td>AS-4CHGL(4-32)</td>
			<td>4% chlorhexidine gluconate surgical hand scrub</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;">Tattoo transfer medium&#160;</td>
			<td style="width:263px;">Hildbrandt Tattoo Supply, Point Roberts, WA</td>
			<td>TRANSF</td>
			<td>Stencil thermal tattoo transfer paper</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Lidocaine with epinephrine</td>
			<td style="width:263px;">ACE Surgical Supply Co, Brockton, MA</td>
			<td style="width:141px;">001-1423</td>
			<td>Lidocaine Hcl 1% (Xylocaine) - Epinephrine 1:100,000, 20ml</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Buprenorphine</td>
			<td style="width:263px;">ZooPharm, Windsor, CO</td>
			<td style="width:141px;"></td>
			<td>1 mg/ml sustained release, dosage 0.01 mg/kg intramuscular</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Digital camera</td>
			<td style="width:263px;">Sony</td>
			<td style="width:141px;">Alpha33&#160;</td>
			<td>Standard digital camera with 18-35mm lens, 3.5-5.6 aperture. Used in automatic mode, no flash</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Tape measure</td>
			<td style="width:263px;">Medline, Mundelein, Illinois</td>
			<td style="width:141px;">NON171330</td>
			<td>Retractable tape measure, cloth, plastic case, 72inches</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Tissue expanders</td>
			<td style="width:263px;">PMT, Chanhassen, MN</td>
			<td style="width:141px;">03610-06-02</td>
			<td>4cm x 6cm, rectangular, 120cc, 3610 series 2 stage tissue expander with standard port</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">ReCap360</td>
			<td style="width:263px;">Autodesk</td>
			<td style="width:141px;">N/A</td>
			<td>MVS Software, Web application: recap360.autodesk.com</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Blender</td>
			<td style="width:263px;">Blender Foundation</td>
			<td style="width:141px;">N/A</td>
			<td>Computer Graphics Software, open source: blender.org</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">SISL</td>
			<td style="width:263px;">SINTEF</td>
			<td style="width:141px;">N/A</td>
			<td>C++ spline libraries, open source: https://www.sintef.no/projectweb/geometry-toolkits/sisl/</td>
		</tr>
	</tbody>
</table>

quantification of strain in a porcine model of skin expansion using multi-view stereo and isogeometric kinematics

Tissue expansion is a popular technique in plastic and reconstructive surgery that grows skin in vivo for correction of large defects such as burns and giant congenital nevi. Despite its widespread use, planning and executing an expansion protocol is challenging due to the difficulty in measuring the deformation imposed at each inflation step and over the length of the procedure. Quantifying the deformation fields is crucial, as the distribution of stretch over time determines the rate and amount of skin grown at the end of the treatment. In this manuscript, we present a method to study tissue expansion in order to gain quantitative knowledge of the deformations induced during an expansion process. This experimental protocol incorporates multi-view stereo and isogeometric kinematic analysis in a porcine model of tissue expansion. Multi-view stereo allows three-dimensional geometric reconstruction from uncalibrated sequences of images. The isogeometric kinematic analysis uses splines to describe the regional deformations between smooth surfaces with few mesh points. Our protocol has the potential to bridge the gap between basic scientific inquiry regarding the mechanics of skin expansion and the clinical setting. Eventually, we expect that the knowledge gained with our methodology will enable treatment planning using computational simulations of skin deformation in a personalized manner.

Denne metode er blevet anvendt til at studere deformationen fremkaldt af forskellige expander geometrier: rektangel, sfære og halvmåne ekspandere 31, 32. Resultaterne svarer til kugle og halvmåne ekspandere diskuteres næste. Figur 2 illustrerer de tre trin af genopbygning MVS model. Udgangspunktet er en samling af fotografier fra en statisk scene. Dyret med de tatoverede net og de målebånd lå stadig som fo...

Watch this Scientific Journal Video about Quantification of Strain in a Porcine Model of Skin Expansion Using Multi-View Stereo and Isogeometric Kinematics at JoVE.com

Quantification of Strain in a Porcine Model of Skin Expansion Using Multi-View Stereo and Isogeometric Kinematics

Denne protokol anvender multi-view stereo til at generere tre-dimensionelle (3D) modeller ud af kalibrerede sekvenser af fotografier, hvilket gør det økonomisk overkommeligt og kan justeres til et kirurgisk indstilling. Strain kort mellem de 3D-modeller er kvantificeret med spline-baserede isogeometric kinematik, som letter repræsentation af glatte overflader end grove masker deler samme parametrering.

Kvantificering af Strain i et porcint Model af hud Ekspansion Brug Multi-View Stereo og Isogeometric Kinematik

Tissue expansion is a popular technique in plastic and reconstructive surgery that grows skin in vivo for correction of large defects such as burns and ...

Research

JoVE Journal

Bioengineering

The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal  Skin and Melanoma Progression

Most in vitro studies in experimental skin biology have been done in 2-dimensional (2D) monocultures, while accumulating evidence suggests that cells ...

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

En fuld huddefekten model til evaluering Vaskularisering biomaterialer<em&gt; In Vivo</em

Insufficient vascularization is considered to be one of the main factors limiting the clinical success of tissue-engineered constructs. In order to ...

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Generation af en tre-dimensionel fuld tykkelse Skin Equivalent og Automated Anskydning

In vitro models are a cost effective and ethical alternative to study cutaneous wound healing processes. Moreover, by using human cells, these models ...

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Forbedring 2D og 3D Skin<em&gt; In vitro</em&gt; Modeller Brug Macromolecular Fortrængning

The glycoprotein family of collagens represents the main structural proteins in the human body, and are key components of biomaterials used in modern ...

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

In Vitro Model af Human Kutan hypertrofisk ardannelse ved hjælp af makromolekylære fortrængning

It has been shown that in vivo tissues are highly crowded by proteins, nucleic acids, ribonucleoproteins, polysaccharides, etc. The following protocol ...

Imaging and Quantification of the Area of Fast-Moving Microbubbles Using a High-Speed Camera and Image Analysis

Billeddannelse og kvantificering af området for hurtige mikrobobler ved hjælp af et højhastighedskamera og billedanalyse

An experimental and image analysis technique is presented for imaging cavitation bubbles and calculating their area. The high-speed imaging experimental ...

Measuring 3D In-vivo Shoulder Kinematics using Biplanar Videoradiography

Måling af 3D In-vivo Skulder kinematics ved hjælp af Biplanar Videoradiography

The shoulder is one of the human body's most complex joint systems, with motion occurring through the coordinated actions of four individual joints, ...

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform

Generering af en forenklet tredimensionel skin-on-a-chip-model i en mikromaskiner med mikrofluidisk platform

This work presents a new, cost-effective, and reliable microfluidic platform with the potential to generate complex multilayered tissues. As a proof of ...

Quantification of Mouse Heart Left Ventricular Function, Myocardial Strain, and Hemodynamic Forces by Cardiovascular Magnetic Resonance Imaging

Kvantificering af mus hjerte venstre ventrikulær funktion, myokardiestamme, og hæmodynamiske kræfter ved hjerte-kar-magnetisk resonans imaging

Mouse models have contributed significantly to understanding genetic and physiological factors involved in healthy cardiac function, how perturbations ...

A Reliable Porcine Fascio-Cutaneous Flap Model for Vascularized Composite Allografts Bioengineering Studies

En pålidelig porcine fascio-kutan klapmodel til vaskulariserede sammensatte allografts bioteknologiske undersøgelser

Vascularized Composite Allografts (VCA) such as hand, face, or penile transplant represents the cutting-edge treatment for devastating skin defects, ...