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DOI :
10.3791/55053-v
February 17th, 2017
Chapters
0:05
Title
0:57
Vascular Cell GFP-TMEM184A Expression and Rhodamine-Heparin Binding
3:42
Fluorescence Resonance Energy Transfer (FRET) from GFP-TMEM184A to Rhodamine-Heparin, and Live Cell Imaging of Rhodamine-Heparin Uptake
5:15
In Vitro Heparin Binding Assay
8:07
Results: Representative GFP-TMEM184A-transfection Effects
9:44
Conclusion
TMEM184A קידוד לבנות עם תג ה- GFP על הסופית- carboxy המיועד ביטוי איקריוטיים, הועסק מבחנים שנועדו לאשר את זיהוי של TMEM184A כמו קולטן הפרין בתאי כלי דם.
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