Name: evaluation of drug sorption to pvc- and non-pvc-based tubes in administration sets using a pump
Uploaded: 2017-03-11T14:00:00
Description: Watch this Scientific Journal Video about Evaluation of Drug Sorption to PVC- and Non-PVC-based Tubes in Administration Sets Using a Pump at JoVE.com

This work was supported by the Korea Ministry of Environment (MOE) as part of the "The advancement of scientific research and technological development in environmental science program (E315-00015-0414-2)."

1.在管理集管的制备注意：正执行切割步骤，以消除对药物吸附在管的长度差异的影响。 <ol><li>使用标记标注与管型的管子的端部（ 例如，聚氯乙烯，聚氨酯（PU），和聚烯烃（PO））。 </li><li>删除所有可拆卸的配件，如连接器和针头盖。 </li><li>用锋利的剃刀，以确保清洁的边缘，从滴注腔的连接切断的管，以1米的长度。 </li></ol> 2.药物注射稀释<...

<ol>
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药物吸附到给药装置在静脉给药意想不到的药物损失的原因。期间吸附，药物通常分配在输液的早期阶段管的聚合物材料;在达到吸附平衡后，药物的递送量被稳定1。药物的吸附量应评估和最小化。药物吸附数的评价方法进行了研究，例如泵的方法和滴注法。相比于滴的方法，该泵的方法可以容易地没有偏压操纵。虽然给药设置与流量调节器（常规形式）在滴注法的使用，所希?...

给药装置是由一个尖峰，一个滴注室，管，鲁尔接头（连接器），以及用于保护针头盖。其他配件，如气道单向阀，一调节夹具，一个在线过滤器，一个Y型管帽（喷射口），和一个针，也可附连到给药装置。药物吸附到管是在注射药物1的递送的一个关键问题。吸附描述了一种药物到聚合物表面和药物的吸收入聚合物基质2的吸附。药物吸附到管在给药装置会导致不可预测的药物损失，使得难以控制递送的药物的浓度。因此，药物吸附到聚合物管是一大障碍的注射药物的精确传递到体内。然而，存在用于药物吸附的评价在施用套管没有标准的方法或法规准则。 <html>药物吸附到管在施用套水平已使用各种评价方法1，2，3，4，5的报道。试验方法和模型药物在吸附评价的关键因素。泵方法1,3和滴注法4,5已被广泛地用于吸附试验。在一般情况下，泵的方法应该在药物与低浓度和低流率作为输注条件的情况下使用。使用各种评价方法，药物吸附的许多研究已经报道了聚氯乙烯（PVC） -和在施用非PVC基管套1，2，3，&gt; 5。许多吸附药物可以选择调查施用套管是否有药物吸附潜力或没有。地西泮（ 图1a）1,2，他克莫司（ 图1b）5，硝酸甘油2和环孢菌素A 3是代表性的药物，在PVC-高吸附和非PVC基管中。 药物吸附在管子的评价，试验条件如流速和药物浓度是基于临床使用所选药物1，6,7。在地西泮的情况下，以1毫升/分钟的流速，使用100微克/毫升的高浓度，以模仿为癫痫持续状态1的治疗的初始剂量。对于他克莫司，10微克/ mL的浓度以10毫升/小时的流速被交付。葡萄糖溶液（5％）用于药物注射的稀释，并且管长为固定在1微米。玻璃瓶和小瓶应当用来防止在实验和贮存过程中附加的吸附。 在这项研究中，我们进行了动力学吸附研究与模型药物，地西泮和他克莫司，用泵方法。该协议的具体细节，从管准备吸附评价，以前描述。药物吸附的评价方法已用于确认注射药物性质和建议的临床使用给药组注射上的情况下，逐案1，2，3，4，5，6，7。这个协议可以被用作标准技术的给药装置吸附评价。药物吸附的评价管国际标准可能是必要的，以确保药物输送的安全性和有效性。

<table>
	<tbody>
		<tr>
			<td>PVC IV sets</td>
			<td>Becton Dickinson 
			(BD) Co. Ltd. (Franklin Lakes, NJ, USA)</td>
			<td></td>
			<td>Internal diameter: 2.54 mm</td>
		</tr>
		<tr>
			<td>PU IV sets</td>
			<td>Tianjin Hanaco Medical (THM) Co.Ltd. 
			(Tianjin, China)</td>
			<td></td>
			<td>Non-PVC 
			Internal diameter: 2.54 mm</td>
		</tr>
		<tr>
			<td>Non-PVC Polyolefin IV sets</td>
			<td>Polyscientech, Co. Ltd (Anseong, Korea)</td>
			<td></td>
			<td>Non-PVC [PE elastomer/PP elastomer/PB elastomer(25/50/25, weight ratio) blend] 
			Internal diameter: 2.54 mm</td>
		</tr>
		<tr>
			<td>Syringe</td>
			<td>Korea Vaccine Co. Ltd. (Seoul, Korea)</td>
			<td>KOVAX-SYRINGE 1 mL 26G 1/2&#34;</td>
			<td></td>
		</tr>
		<tr>
			<td>Daewon diazepam injection</td>
			<td>Daewon Pharma. Co. Ltd. (Hwaseong, Gyunggi, Korea)</td>
			<td></td>
			<td>1 mg/mL, total 2 mL 
			Batch No.: P003 
			Composition: diazepam, propylene glycol, ethanol, benzyl alcohol, sodium benzoate, bezoic acid, water for injection</td>
		</tr>
		<tr>
			<td>Tacrobel injection</td>
			<td>Chong Keun Dang, Co. Ltd. (Seoul, Korea)</td>
			<td></td>
			<td>5 mg/mL, total 1 mL 
			Batch No.: AG002 
			Composition: tacrolimus hydrate, polyoxyl 60 hydrogenated castor oil (HCO-60), dehydrated alcohol</td>
		</tr>
		<tr>
			<td>5% Dextrose</td>
			<td>JW Pharmaceutical (Seoul, Korea)</td>
			<td>500 mL</td>
			<td></td>
		</tr>
		<tr>
			<td>5% Dextrose</td>
			<td>Daehan Pharmaceutical (Seoul, Korea)</td>
			<td>200 mL</td>
			<td>Bottle (glass)</td>
		</tr>
		<tr>
			<td>Amber vials</td>
			<td></td>
			<td>20 mL</td>
			<td>Glass</td>
		</tr>
		<tr>
			<td>Terumo infusion pump</td>
			<td>Terumo (Medical Corp., USA)</td>
			<td>TE-135</td>
			<td></td>
		</tr>
		<tr>
			<td>HPLC system with UV detector</td>
			<td>Agilent (Santa Clara, CA, USA)</td>
			<td>Agilent 1260</td>
			<td></td>
		</tr>
		<tr>
			<td>CAPCELL PAK C18 column&#160;</td>
			<td>Shiseido (Japan)</td>
			<td>90404</td>
			<td>1.5 mm x 250 mm, 5 &#956;m</td>
		</tr>
		<tr>
			<td>Diazepam</td>
			<td></td>
			<td></td>
			<td>From Daewon Pharma. Co., Ltd.</td>
		</tr>
		<tr>
			<td>Tacrolimus</td>
			<td>Teva Czech industries&#160; (Czech Republic)</td>
			<td></td>
			<td>From Chong Keun Dang, Co. Ltd.</td>
		</tr>
		<tr>
			<td>Acetonitrile</td>
			<td>Burdick and Jackson Co., Ltd. (MI, USA)</td>
			<td>3/1/9017</td>
			<td></td>
		</tr>
		<tr>
			<td>Methanol</td>
			<td>Burdick and Jackson Co. Ltd. (MI, USA)</td>
			<td>AH230-4</td>
			<td></td>
		</tr>
		<tr>
			<td>Water</td>
			<td>Burdick and Jackson Co. Ltd. (MI, USA)</td>
			<td>3/1/4218</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium dihydrogen phosphate</td>
			<td>Sigma (St. Louis, MO, USA)</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Phosphoric acid</td>
			<td>Sigma (St. Louis, MO, USA)</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

evaluation of drug sorption to pvc- and non-pvc-based tubes in administration sets using a pump

管理组是直接应用的药物进入体内交付工具和由一个尖峰，点滴室，管，鲁尔适配器（连接器），用于保护针头帽，配件等。药物吸附到施用套管是在安全性和有效性方面的关键问题。虽然药物吸附是在一个管理集的质量的一个重要因素，存在用于药物吸附在管子的调节没有标准的评价方法。在这里，我们描述了药物吸收的评估协议的管理组管。制成的聚氯乙烯（PVC）管 - 和非PVC基聚合物材料被切成1米的长度。地西泮和他克莫司被用作模型药物。在动力学吸附研究中，我们选择了基于这些药物的临床使用的药物浓度和流速。在玻璃瓶中每种药物的稀释后，将稀释的药物溶液通过管输送施用的设置使用的泵。样品收集在琥珀色小瓶在适当的时间点，并使用高效液相色谱法进行分析的药物。药物浓度和吸附各级行政套管进行了计算。建议上可接受的标准，以确保的给药装置的质量。

吸附在给药组管使用模型药物，地西泮（ 图1a）和他克莫司被动力学监测（ 图1b），和泵的方法（ 图2）。稀释药物（ 图2a）的混合物在使用输液泵（ 图2c）的固定流动速率（ 图2b图 ）通过PVC-和非基于PVC的管子通过。玻璃瓶被稍微打开以允许施用集管的插入。该药物是通过管（ 图2d）</stron...

Watch this Scientific Journal Video about Evaluation of Drug Sorption to PVC- and Non-PVC-based Tubes in Administration Sets Using a Pump at JoVE.com

Evaluation of Drug Sorption to PVC- and Non-PVC-based Tubes in Administration Sets Using a Pump

我们报告使用泵和模型药物（地西泮和他克莫司），用于药物吸附的评价方法。后用高效液相色谱法，药物浓度和吸附水平的施用套管药物分析计算。

药物吸附的评价PVC-和非PVC为基础的管理管设置在使用泵

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Intranasal administration has been reported to be a potential pathway for nose-to-brain delivery of therapeutic agents that circumvents the blood-brain ...

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